Pirtle R, Kashdan M, Pirtle I, Dudock B
Nucleic Acids Res. 1980 Feb 25;8(4):805-15.
Through the use of a variety of post-labeling techniques, the nucleotide sequence of a major species of leucine tRNA from bovine liver was determined to be pG-G-U-A-G-C-G-U-G-m-G-C-ac-C-G-A-G-C-G-G-D-C-psi-A-A-G-G-C-m-G-C-U-G-G-A-psim- U-I-A-G-m-G-C-psi-C-C-A-G-U-C-psi-C-psi-U-C-G-G-G-G-G-m-C-G-U-G-G-G-T-psi-C-G-m -A-A-U-C-C-C-A-C-C-G-C-U-G-C-C-A-C-C-AOH. A comparison of known sequences of leucine tRNAs shows a consistent set of features which clearly distinguish prokaryotic and eukaryotic leucine tRNAs from each other.
通过使用多种标记后技术,确定来自牛肝的亮氨酸tRNA主要种类的核苷酸序列为pG-G-U-A-G-C-G-U-G-m-G-C-ac-C-G-A-G-C-G-G-D-C-psi-A-A-G-G-C-m-G-C-U-G-G-A-psim-U-I-A-G-m-G-C-psi-C-C-A-G-U-C-psi-C-psi-U-C-G-G-G-G-G-m-C-G-U-G-G-G-T-psi-C-G-m-A-A-U-C-C-C-A-C-C-G-C-U-G-C-C-A-C-C-AOH。对已知亮氨酸tRNA序列的比较显示出一组一致的特征,这些特征清楚地将原核生物和真核生物的亮氨酸tRNA区分开来。