Sprouse H M, Kashdan M, Otis L, Dudock B
Nucleic Acids Res. 1981 Jun 11;9(11):2543-7. doi: 10.1093/nar/9.11.2543.
The nucleotide sequence of a spinach chloroplast valine tRNA (sp. chl. tRNA Val) has been determined. This tRNA shows essentially equal homology to prokaryotic valine tRNAs (58-65% homology) and to the mitochondrial valine tRNAs of lower eukaryotes (yeast and N. crassa, 61-62% homology). Sp. chl. tRNA Val shows distinctly lower homology to mouse mitochondrial valine tRNA (53% homology) and to eukaryotic cytoplasmic valine tRNAs (47-53% homology). Sp. chl. tRNA Val, like all other chloroplast tRNAs sequenced, contains a methylated GG sequence in the dihydrouridine loop and lacks unusual structural features which have been found in several mitochondrial tRNAs.
已确定菠菜叶绿体缬氨酸tRNA（sp. chl. tRNA Val）的核苷酸序列。该tRNA与原核缬氨酸tRNA的同源性基本相同（58 - 65%同源性），与低等真核生物（酵母和粗糙脉孢菌）的线粒体缬氨酸tRNA的同源性也基本相同（61 - 62%同源性）。Sp. chl. tRNA Val与小鼠线粒体缬氨酸tRNA的同源性明显较低（53%同源性），与真核细胞质缬氨酸tRNA的同源性也较低（47 - 53%同源性）。与所有其他已测序的叶绿体tRNA一样，Sp. chl. tRNA Val在二氢尿嘧啶环中含有一个甲基化的GG序列，并且缺乏在几种线粒体tRNA中发现的异常结构特征。
