温度对流感病毒神经氨酸酶和核蛋白基因在不含变性剂的丙烯酰胺凝胶中电泳迁移顺序的影响。
Effect of temperature on the order of electrophoretic migration of influenza virus neuraminidase and nucleoprotein genes in acrylamide gels lacking denaturing agents.
作者信息
Cox N J, Kendal A P
出版信息
J Gen Virol. 1978 Jul;40(1):229-32. doi: 10.1099/0022-1317-40-1-229.
PMID:690606
Abstract
When subjected to electrophoresis at 33 degrees C in 3% polyacrylamide gels with no urea added, the nucleoprotein and neuraminidase genes of an H2N2 and H3N2 virus migrate as RNA bands 5 and 6 respectively. If the temperature of electrophoresis is increased to 46 degrees C, however, this order of migration is reversed.
摘要
当在不添加尿素的3%聚丙烯酰胺凝胶中于33摄氏度进行电泳时，H2N2和H3N2病毒的核蛋白基因和神经氨酸酶基因分别作为RNA条带5和条带6迁移。然而，如果将电泳温度提高到46摄氏度，这种迁移顺序就会颠倒。
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