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流感病毒基因组图谱：血凝素和神经氨酸酶基因的鉴定。

Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes.

作者信息

Palese P, Schulman J L

出版信息

Proc Natl Acad Sci U S A. 1976 Jun;73(6):2142-6. doi: 10.1073/pnas.73.6.2142.

DOI:10.1073/pnas.73.6.2142
PMID:1064882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430466/
Abstract

Polyacrylamide gel electrophoresis of the RNA of influenza A/PR/8/34 (H0N1) and A/Hong Kong/8/68 (H3N2) viruses and recombinant viruses derived from them revealed that each contains eight RNA segments, the fourth of which codes for hemagglutinin. (The largest RNA of the segmented genome is counted as band 1.) The neuraminidase gene was identified as the sixth segment in the RNA pattern of influenza A/PR8 virus and as the fifth segment of A/Hong Kong virus. The molecular weights of the RNAs for the hemagglutinin and the neuraminidase genes lie in the range of 600,000-700,000.

摘要

对甲型流感病毒A/PR/8/34（H0N1）、A/香港/8/68（H3N2）及其衍生的重组病毒的RNA进行聚丙烯酰胺凝胶电泳分析，结果显示，每种病毒均含有8个RNA片段，其中第4个片段编码血凝素。（分段基因组中最大的RNA计为条带1。）在甲型流感病毒A/PR8的RNA图谱中，神经氨酸酶基因被鉴定为第6个片段，而在甲型流感病毒A/香港的RNA图谱中，该基因则为第5个片段。血凝素基因和神经氨酸酶基因的RNA分子量在600,000 - 700,000范围内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c98/430466/ce84f5484898/pnas00036-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c98/430466/2c7419ae9dcc/pnas00036-0377-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c98/430466/cd44ba501b16/pnas00036-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c98/430466/ce84f5484898/pnas00036-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c98/430466/2c7419ae9dcc/pnas00036-0377-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c98/430466/cd44ba501b16/pnas00036-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c98/430466/ce84f5484898/pnas00036-0379-a.jpg

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