Dimmock N J, Carver A S, Kennedy S I, Lee M R, Luscombe S
J Gen Virol. 1977 Sep;36(3):503-13. doi: 10.1099/0022-1317-36-3-503.
Methods are described for the preparation in vivo of 35S-methionine-labelled influenza viruses, the purifiction of the nucleoprotein (NP) and matrix (M) proteins and the separation of peptides obtained by protease digestion by two-dimensional thin-layer chromatography. The maps of the M proteins of A/Okuda/57(H2N2) and A/Finland/4/74(H3N2) were very similar overall but differed in three peptides. Hence they could be clearly distinguished. Maps of the NP proteins of the same strains showed a greater number of differences. A recombinant strain having the haemagglutinin and neuraminidase of the A/Finland/4/74 parent and the virulence of the A/Okuda/57 parent was shown to have the M and P proteins of A/okuda/57.
本文描述了在体内制备35S-甲硫氨酸标记的流感病毒、纯化核蛋白（NP）和基质（M）蛋白以及通过二维薄层色谱分离蛋白酶消化获得的肽段的方法。A/奥久田/57（H2N2）和A/芬兰/4/74（H3N2）的M蛋白图谱总体上非常相似，但在三个肽段上有所不同。因此，它们可以被清楚地区分。相同毒株的NP蛋白图谱显示出更多差异。一种重组毒株具有A/芬兰/4/74亲本的血凝素和神经氨酸酶以及A/奥久田/57亲本的毒力，结果表明它具有A/奥久田/57的M蛋白和P蛋白。
