Amemiya T
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1981;216(2):103-9. doi: 10.1007/BF00414577.
The photoreceptor outer segments and retinal pigment epithelium of vitamin E deficient rats were examined by electron microscope using electron histochemical techniques. In rats fed a vitamin E deficient diet for more than 6 months starting 20 days after birth, the photoreceptor outer segment showed vesiculation of the discs near the apical portion of the retinal pigment epithelium. In the retinal pigment epithelium, secondary lysosomes were increased in number. Reaction products of acid phosphatase activity were located in secondary lysosomes in the retinal pigment epithelium and on the disc membranes of the photoreceptor outer segment. These alterations became more prominent with the duration of vitamin E deficiency. It was assumed that the disc degeneration of the photoreceptor outer segment might be due to enhanced activity of lysosomal enzymes in the retinal pigment epithelium and the effect of released lysomal enzymes on the photoreceptor outer segment.
采用电子组织化学技术，通过电子显微镜对维生素E缺乏大鼠的光感受器外段和视网膜色素上皮进行了检查。在出生后20天开始喂食维生素E缺乏饮食超过6个月的大鼠中，光感受器外段在视网膜色素上皮顶端部分附近的圆盘出现了囊泡化。在视网膜色素上皮中，次级溶酶体数量增加。酸性磷酸酶活性的反应产物位于视网膜色素上皮的次级溶酶体和光感受器外段的圆盘膜上。随着维生素E缺乏时间的延长，这些改变变得更加明显。据推测，光感受器外段的圆盘退化可能是由于视网膜色素上皮中溶酶体酶活性增强以及释放的溶酶体酶对光感受器外段的作用所致。
