Amemiya T
Int J Vitam Nutr Res. 1981;51(2):114-8.
The photoreceptor outer segment and retinal pigment epithelium of vitamin E deficient rats given vitamin E were studied by electron microscopy. In rats fed a vitamin E deficient diet for more than 12 months, the photoreceptor outer segment did not regenerate after the administration of 400 mg of vitamin E. The retinal pigment epithelium was filled with lipofuscin granules and contained cytoplasmic organelles such as mitochondria, ribosomes and Golgi apparatus. The microvilli of the retinal pigment epithelium were long and in contact with the outer nuclear layer cells. The photoreceptor inner segment and nucleus had almost disappeared. In rats given 400 mg of vitamin E after vitamin E deficiency for 6 months, the photoreceptor outer segment showed no vesiculation and were increased in number. However, some retinal pigment epithelial cells still contained many lipofuscin granules. In conclusion, the photoreceptor outer segment and retinal pigment epithelium can be repaired in mild vitamin E deficiency by vitamin E administration, but vitamin E cannot reproduce the photoreceptor cells in nuclei of which have been broken by severe vitamin E deficiency.
通过电子显微镜研究了给予维生素E的维生素E缺乏大鼠的光感受器外段和视网膜色素上皮。在喂食维生素E缺乏饮食超过12个月的大鼠中，给予400毫克维生素E后，光感受器外段未再生。视网膜色素上皮充满脂褐素颗粒，并含有线粒体、核糖体和高尔基体等细胞质细胞器。视网膜色素上皮的微绒毛很长，与外核层细胞接触。光感受器内段和细胞核几乎消失。在维生素E缺乏6个月后给予400毫克维生素E的大鼠中，光感受器外段未出现囊泡化，数量增加。然而，一些视网膜色素上皮细胞仍含有许多脂褐素颗粒。总之，在轻度维生素E缺乏时，给予维生素E可修复光感受器外段和视网膜色素上皮，但维生素E不能再生因严重维生素E缺乏而细胞核已受损的光感受器细胞。
