Kashdan M A, Dudock B S
J Biol Chem. 1982 Oct 10;257(19):11191-4.
The nucleotide sequence of the gene for spinach chloroplast tRNAIle1 has been determined. The gene is found in two copies located in the inverted repeat regions of spinach chloroplast DNA, but not within the ribosomal RNA spacer. Both copies of the tRNAIle1 gene have been sequenced and found to be identical. A very unusual characteristic of the tRNAIle1 gene is that the anticodon is CAT which is a methionine anticodon. In the tRNA the C residue in the anticodon is subsequently modified, presumably to prevent misreading of the genetic code. The spinach chloroplast tRNAIle1 gene is colinear with its RNA sequence and does not contain an intervening sequence as has been reported for maize chloroplast tRNAIle2 (Koch, W., Edwards, K., and Kossel, H. (1981) Cell 25, 203-213). The tRNAIle1 gene does not code for the 3'-terminal CCA end, nor do any other tRNA genes appear to be contiguous with this gene.
菠菜叶绿体异亮氨酸tRNA1（tRNAIle1）基因的核苷酸序列已被确定。该基因以两个拷贝的形式存在于菠菜叶绿体DNA的反向重复区域中，但不在核糖体RNA间隔区内。tRNAIle1基因的两个拷贝均已测序，结果发现它们是相同的。tRNAIle1基因一个非常不寻常的特征是其反密码子为CAT，而这是一个甲硫氨酸的反密码子。在该tRNA中，反密码子中的C残基随后会被修饰，推测这是为了防止遗传密码的错读。菠菜叶绿体tRNAIle1基因与其RNA序列是共线性的，并且不像玉米叶绿体异亮氨酸tRNA2（Koch, W., Edwards, K., and Kossel, H. (1981) Cell 25, 203 - 213）那样含有间隔序列。tRNAIle1基因不编码3'-末端的CCA序列，似乎也没有任何其他tRNA基因与该基因相邻。
