Pflugradt K, Voss C, Hartmann N
Nahrung. 1978;22(2):229-36. doi: 10.1002/food.19780220214.
The effect of a high-fat diet on the diameter, volume, triglyceride content and number of the adipocytes was studied in male Wistar rats at the age of 9 weeks and 6 months, respectively. The adipocytes were isolated by means of the method of RODBELL. The adipocyte diameters were measured using the microscopic method. The weight and the fat content of the fatty tissue were determined gravimetrically. A diet containing 50% fat increases the diameter, volume and triglyceride content of the adipocytes and also the weight and the fat content of the different types of fatty tissue. The fat content of the diet and the duration of feeding are of importance. The high-fat diet exerts no effect on the adipocyte number. However, in both age groups, the adipocyte numbers were sometimes greater in heavy animals than in light ones fed the same diet. Genetic, hormonal, and dietarily-induced factors are considered as a possible cause.
分别在9周龄和6月龄的雄性Wistar大鼠中研究了高脂饮食对脂肪细胞直径、体积、甘油三酯含量及数量的影响。采用RODBELL法分离脂肪细胞。使用显微镜法测量脂肪细胞直径。通过重量法测定脂肪组织的重量和脂肪含量。含50%脂肪的饮食会增加脂肪细胞的直径、体积和甘油三酯含量，以及不同类型脂肪组织的重量和脂肪含量。饮食中的脂肪含量和喂养持续时间很重要。高脂饮食对脂肪细胞数量没有影响。然而，在两个年龄组中，体重较重的动物的脂肪细胞数量有时比喂食相同饮食的体重较轻的动物更多。遗传、激素和饮食诱导因素被认为是可能的原因。
