血清因子改变了正常人成纤维细胞增殖对钙离子、钾离子、镁离子、磷酸根离子和2-氧代羧酸的细胞需求。
Serum factors modify the cellular requirement for Ca2+, K+, Mg2+, phosphate ions, and 2-oxocarboxylic acids for multiplication of normal human fibroblasts.
作者信息
McKeehan W L, McKeehan K A
出版信息
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3417-21. doi: 10.1073/pnas.77.6.3417.
The rate of multiplication of a population of cultured human lung fibroblasts is determined by the level of common nutrients and serum factors in the medium. By application of the principles of Henri--Michaelis--Menten kinetic analysis to cell growth in vitro, the relationship between the level of a macromolecular fraction of serum that contains growth factors and the concentration of individual nutrient that is required to support a half-maximal rate of cell multiplication was explored. The requirement for the majority of individual nutrients is independent of the level of serum factors in the medium. This includes all 20 amino acids, glucose, purines, pyrimidines, polyamines, choline, and inositol. In contrast, serum factors determine the cellular requirement for Ca2+, K+, Mg2+, Pi, and 2-oxocarboxylic acids. These nutrients are most likely involved in cellular processes related to the mechanism by which growth factors in serum control the cell multiplication rate.
培养的人肺成纤维细胞群体的增殖速率由培养基中常见营养素和血清因子的水平决定。通过将亨利 - 米氏动力学分析原理应用于体外细胞生长，探讨了含有生长因子的血清大分子组分水平与支持细胞增殖半最大速率所需的单个营养素浓度之间的关系。大多数单个营养素的需求与培养基中血清因子的水平无关。这包括所有20种氨基酸、葡萄糖、嘌呤、嘧啶、多胺、胆碱和肌醇。相比之下，血清因子决定了细胞对Ca2 +、K +、Mg2 +、Pi和2 - 氧代羧酸的需求。这些营养素很可能参与了与血清中生长因子控制细胞增殖速率机制相关的细胞过程。
Zotero 插件