Kal H B, Barendsen G W
Br J Cancer Suppl. 1980 Apr;4:275-8.
The rat R-1 rhabdomyosarcoma with a capacity for colony growth in vitro after excision of the tumour and dissociation by a trypsin method has been used to investigate the effectiveness of radio-chemotherapy. Growth delay data were compared with data on survival of cells derived from tumours treated in situ. An excess in growth delay was observed when vinblastine (1.5 mg/kg) was given at intervals of 0.3 to 2 d after or 4 d before a dose of 20 Gy of X-rays. Cell survival data indicate that the maximum effectiveness of the drug treatment and the combined treatment (vinblastine and a dose of 10 Gy) can be assessed 2 to 3 d after treatment. The fractions of surviving cells determined after combined therapy at 0, 1 and 2 d intervals were not significantly different from the fractions expected on the basis of simple multiplication of the fractions surviving individual treatments. The data suggest that the excess in tumour growth delay observed cannot be accounted for by co-operative interaction of the doses of radiation and drug.
通过胰蛋白酶法切除肿瘤并解离后，具有体外集落生长能力的大鼠R-1横纹肌肉瘤已被用于研究放化疗的有效性。将生长延迟数据与原位治疗肿瘤来源细胞的存活数据进行比较。当在20 Gy X射线剂量后0.3至2天或前4天间隔给予长春碱（1.5 mg/kg）时，观察到生长延迟增加。细胞存活数据表明，药物治疗和联合治疗（长春碱和10 Gy剂量）的最大有效性可在治疗后2至3天评估。联合治疗后每隔0、1和2天测定的存活细胞分数与基于单独治疗存活分数简单相乘预期的分数无显著差异。数据表明，观察到的肿瘤生长延迟增加不能用辐射剂量和药物的协同相互作用来解释。
