Gauwerky C, Golde D W
Blood. 1980 Nov;56(5):886-91.
We have investigated the hormonal responsiveness of K562 cells using a serum-substituted in vitro clonogenic assay. Dexamethasone inhibited colony formation by the K562 cells, and the inhibitory effect could be reversed by progesterone (10(-6) M). Fluoxymesterone caused a prominent enhancement of K562 colony growth, whereas estriol had no effect. Stimulation by triiodothyronine was maximal at 10(-7) M, and the thyroid effect could be abrogated by the beta 2-adrenergic antagonist butoxamine in equimolar concentrations. Using standard tissue culture conditions, the beta-adrenergic agent isoproterenol, but not the alpha catecholamine phenylephrine, enhanced the proliferation of K562 cells. When K562 cells were grown under hormone-depleted conditions, they developed responsiveness to phenylephrine and were no longer stimulated by isoproterenol. DbcAMP and prostaglandins of the E series also caused K562 colony enhancement. Prostaglandin F2 alpha had no effect on cell proliferation. Insulin was an effective stimulant of colony formation of K562 cells, as were human growth hormone and ovine prolacin. Bovine growth hormone had no effect. Our results are consistent with the identificaiton of K562 as an erythroid line, and they indicate that K562 cells respond to endocrine hormones in a manner analogous to normal erythroid progenitors.
我们使用血清替代的体外克隆形成试验研究了K562细胞的激素反应性。地塞米松抑制K562细胞的集落形成，而孕酮（10⁻⁶ M）可逆转这种抑制作用。氟甲睾酮显著促进K562细胞集落生长，而雌三醇则无作用。三碘甲状腺原氨酸在10⁻⁷ M时刺激作用最大，且甲状腺效应可被等摩尔浓度的β₂-肾上腺素能拮抗剂布托沙明消除。在标准组织培养条件下，β-肾上腺素能药物异丙肾上腺素而非α-儿茶酚胺去氧肾上腺素可增强K562细胞的增殖。当K562细胞在激素缺乏条件下生长时，它们对去氧肾上腺素产生反应，且不再受异丙肾上腺素刺激。环磷腺苷（dbcAMP）和E系列前列腺素也可促进K562细胞集落生长。前列腺素F2α对细胞增殖无作用。胰岛素是K562细胞集落形成的有效刺激物，人生长激素和羊催乳素也是如此。牛生长激素无作用。我们的结果与将K562鉴定为红系细胞系一致，并且表明K562细胞对内分泌激素的反应方式类似于正常红系祖细胞。
