Westin E H, Gallo R C, Arya S K, Eva A, Souza L M, Baluda M A, Aaronson S A, Wong-Staal F
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2194-8. doi: 10.1073/pnas.79.7.2194.
Total cellular RNAs from a variety of fresh and culture-derived human hematopoietic neoplastic cell types at various stages of differentiation and human sarcoma, carcinoma, melanoma, and glioblastoma cell lines were enriched for poly(A)- containing sequences, fractionated by gel electrophoresis, and blot hybridized to a cloned DNA probe containing the transforming sequences (v-amv) of avian myeloblastosis virus (AMV), a virus known to cause myeloid leukemias in chickens. Expression of RNA sequences homologous to AMV was detected in all immature myeloid and lymphoid T cells in addition to the single erythroid cell line examined, but not in mature T cells or in B cells, including lymphoblast cell lines derived from patients with Burkitt lymphoma. In addition, induction of the cell line HL60, a promyelocytic leukemia line, to differentiate with dimethyl sulfoxide or retinoic acid resulted in a reduction of the level of expression of the human cellular gene c-amv homologous to v-amv. There was no detectable c-amv mRNA in any of the solid tumor cell lines examined. Thus, expression of the human c-amv gene could be correlated with the stage of differentiation of different hematopoietic cell types determined by morphologic and marker studies. Expression of c-amv could not be correlated with the extent of methylation in HL60 and in HL60 induced to differentiate with dimethyl sulfoxide.
从处于不同分化阶段的多种新鲜的和培养来源的人类造血肿瘤细胞类型以及人类肉瘤、癌、黑色素瘤和胶质母细胞瘤细胞系中提取的总细胞RNA，富集含多聚腺苷酸的序列，通过凝胶电泳进行分级分离，然后与一个含有禽成髓细胞瘤病毒（AMV）转化序列（v - amv）的克隆DNA探针进行印迹杂交，AMV是一种已知可在鸡中引起髓性白血病的病毒。除了所检测的单个红系细胞系外，在所有未成熟髓系和淋巴T细胞中均检测到与AMV同源的RNA序列的表达，但在成熟T细胞或B细胞中未检测到，包括源自伯基特淋巴瘤患者的淋巴母细胞系。此外，用二甲基亚砜或视黄酸诱导早幼粒细胞白血病细胞系HL60分化，导致与v - amv同源的人类细胞基因c - amv的表达水平降低。在所检测的任何实体瘤细胞系中均未检测到c - amv mRNA。因此，人类c - amv基因的表达可与通过形态学和标志物研究确定的不同造血细胞类型的分化阶段相关联。c - amv的表达与HL60以及用二甲基亚砜诱导分化的HL60中的甲基化程度无关。
