Bennett M, Schmid K
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6109-13. doi: 10.1073/pnas.77.10.6109.
Human plasma alpha 1-acid glycoprotein (orosomucoid) and derivatives of this protein produced by sequential enzymatic cleavage of the glycosidic residue (sialic acid, galactose, N-acetylglucosamine, mannose) were tested for the ability suppress a number of immune functions of mouse spleen cells in vitro at physiological concentrations. alpha 1-Acid glycoprotein and especially the agalacto/asialo derivative suppressed (i) the mitogenic responses to concanavalin A, lipopolysaccharide, and alloantigens, (ii) antibody responses to sheep erythrocytes, and (iii) induction of cell-mediated lympholysis against allogeneic target cells. The native protein and its derivative did not inhibit the proliferation of EL-4 lymphoma cells and did not suppress the lysis of YAC-1 lymphoma cells by natural killer cells. The enhanced potency of the agalacto/asialo derivative indicates that the nature of the carbohydrate moiety exposed on the protein determines the effectiveness of the glycoprotein and its deglycosylated derivatives may function to regulate immune responses in various physiological and pathological conditions.
对人血浆α1-酸性糖蛋白（类粘蛋白）及其通过糖苷残基（唾液酸、半乳糖、N-乙酰葡糖胺、甘露糖）的顺序酶切产生的该蛋白衍生物，在生理浓度下于体外抑制小鼠脾细胞多种免疫功能的能力进行了测试。α1-酸性糖蛋白，尤其是去半乳糖/去唾液酸衍生物抑制了：（i）对伴刀豆球蛋白A、脂多糖和同种异体抗原的促有丝分裂反应，（ii）对绵羊红细胞的抗体反应，以及（iii）针对同种异体靶细胞的细胞介导淋巴细胞溶解的诱导。天然蛋白及其衍生物不抑制EL-4淋巴瘤细胞的增殖，也不抑制自然杀伤细胞对YAC-1淋巴瘤细胞的裂解。去半乳糖/去唾液酸衍生物增强的效力表明，蛋白上暴露的碳水化合物部分的性质决定了糖蛋白的有效性，其去糖基化衍生物可能在各种生理和病理条件下起调节免疫反应的作用。
