Sugden P J, Lilleyman J S
Br J Haematol. 1980 Nov;46(3):367-75. doi: 10.1111/j.1365-2141.1980.tb05983.x.
In vitro incorporation of tritiated thymidine was measured in blast cells from 33 unselected and untreated patients with lymphoblastic leukaemia (ALL) both when autologous plasma was present and also when it was substituted by pooled normal plasma. A similar procedure was carried out on 19 patients with acute non-lymphoblastic leukaemia (AnonLL). Plasma from the patients with ALL was variably found to reduce the uptake of the radiolabelled DNA-precursor by their own blasts, with the most marked effect apparent in those with shorter survival times (P < 0.02). This phenomenon was not observed in AnonLL. The amount of plasma-mediated inhibition shown by the ALL patients also correlated overall with their WBC, a known feature of a poor outlook, but even in the 21 patients with WBC below 20.0 x 10(9)/l there was still a significant association between plasma effect and survival (P < 0.01). The demonstration of such inhibitory plasma may be a useful prognostic marker in otherwise 'standard risk' ALL, and also may indicate a large tumour mass where this is not reflected by the height of the circulating WBC.
在33例未经挑选和治疗的淋巴细胞白血病（ALL）患者的原始细胞中，测定了氚标记胸腺嘧啶核苷的体外掺入情况，实验分别在存在自体血浆以及用混合正常血浆替代自体血浆时进行。对19例急性非淋巴细胞白血病（AnonLL）患者也进行了类似操作。结果发现，ALL患者的血浆对其自身原始细胞摄取放射性标记的DNA前体有不同程度的降低作用，在生存期较短的患者中这种作用最为明显（P<0.02）。在AnonLL患者中未观察到这种现象。ALL患者血浆介导的抑制作用程度总体上也与其白细胞计数相关，白细胞计数是预后不良的一个已知特征，但即使在21例白细胞计数低于20.0×10⁹/L的患者中，血浆效应与生存期之间仍存在显著关联（P<0.01）。这种抑制性血浆的证实可能是“标准风险”ALL中一个有用的预后标志物，并且也可能表明存在较大肿瘤肿块，而这在循环白细胞数量上并未体现出来。
