人类淋巴母细胞含有DNA糖基化酶活性，可切除N-3和N-7甲基及乙基嘌呤，但不能切除O6-烷基鸟嘌呤或1-烷基腺嘌呤。
Human lymphoblasts contain DNA glycosylase activity excising N-3 and N-7 methyl and ethyl purines but not O6-alkylguanines or 1-alkyladenines.
作者信息
Singer B, Brent T P
出版信息
Proc Natl Acad Sci U S A. 1981 Feb;78(2):856-60. doi: 10.1073/pnas.78.2.856.
Abstract
Cultured human lymphoblasts (CCRF-CEM line) have DNA glycosylase activities, the specificities of which were investigated by using high-performance liquid chromatography. In addition to 3-methyladenine, 3-ethyladenine, 7-methylguanine, 7-ethylguanine, 3-methylguanine, and 3-ethylguanine also were excised from alkylated double-stranded DNA and deoxypolynucleotides, but 1-methyladenine, 1-ethyladenine, O6-methylguanine, and O6-ethylguanine were not. The glycosylase activity was generally greater for the methylated than for the corresponding ethylated purines and was also greater toward 3-alkyladenine than toward 3-alkyladenine than toward 3-alkylguanine. 7-Methylguanine and 7-ethylguanine were excised to similar but low extents. However, in molar terms, the release of 7-methylguanine was similar to that of 3-methyladenine.
摘要
培养的人淋巴母细胞（CCRF - CEM 细胞系）具有 DNA 糖基化酶活性，利用高效液相色谱法对其特异性进行了研究。除了 3 - 甲基腺嘌呤外，3 - 乙基腺嘌呤、7 - 甲基鸟嘌呤、7 - 乙基鸟嘌呤、3 - 甲基鸟嘌呤和 3 - 乙基鸟嘌呤也能从烷基化双链 DNA 和脱氧多核苷酸中被切除，但 1 - 甲基腺嘌呤、1 - 乙基腺嘌呤、O6 - 甲基鸟嘌呤和 O6 - 乙基鸟嘌呤则不能。对于甲基化嘌呤，糖基化酶活性通常比对相应的乙基化嘌呤更高，并且对 3 - 烷基腺嘌呤的活性比对 3 - 烷基鸟嘌呤的活性也更高。7 - 甲基鸟嘌呤和 7 - 乙基鸟嘌呤被切除的程度相似但较低。然而，以摩尔计，7 - 甲基鸟嘌呤的释放量与 3 - 甲基腺嘌呤的释放量相似。
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Human lymphoblasts contain DNA glycosylase activity excising N-3 and N-7 methyl and ethyl purines but not O6-alkylguanines or 1-alkyladenines.人类淋巴母细胞含有DNA糖基化酶活性，可切除N-3和N-7甲基及乙基嘌呤，但不能切除O6-烷基鸟嘌呤或1-烷基腺嘌呤。
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