啮齿动物肝脏提取物对甲基化DNA中7-甲基鸟嘌呤的酶促释放。
Enzymatic release of 7-methylguanine from methylated DNA by rodent liver extracts.
作者信息
Margison G P, Pegg A E
出版信息
Proc Natl Acad Sci U S A. 1981 Feb;78(2):861-5. doi: 10.1073/pnas.78.2.861.
Abstract
Rat and hamster liver extracts were found to contain DNA glycosylases capable of removing 3-methyladenine and 7-methylguanine from methylated DNA. The activity of 7-methylguanine-DNA glycosylase was greater tin hamster than in rat liver extracts. This finding is consistent with previous reports that the half-life of 7-methylguanine in DNA after treatment with the carcinogen dimethylnitrosamine is longer in rats than in hamsters. These enzymes may, therefore, play an important role in the removal of abnormal alkylation products from mammalian cell DNA. Rodent liver extracts also contained a DNA glycosylase able to remove from alkylated DNA the imidazole-ring-opened form of 7-methylguanine which is produced by treatment with alkali. Although this product may occur in vivo after treatment with alkylating agents to only a very small extent, the enzyme may be needed to minimize its potentially harmful biological effects.
摘要
研究发现，大鼠和仓鼠肝脏提取物中含有能够从甲基化DNA中去除3-甲基腺嘌呤和7-甲基鸟嘌呤的DNA糖基化酶。仓鼠肝脏提取物中7-甲基鸟嘌呤-DNA糖基化酶的活性高于大鼠肝脏提取物。这一发现与之前的报道一致，即在用致癌物二甲基亚硝胺处理后，大鼠DNA中7-甲基鸟嘌呤的半衰期比仓鼠长。因此，这些酶可能在从哺乳动物细胞DNA中去除异常烷基化产物方面发挥重要作用。啮齿动物肝脏提取物还含有一种DNA糖基化酶，能够从烷基化DNA中去除经碱处理产生的7-甲基鸟嘌呤的咪唑环开环形式。尽管这种产物在用烷基化剂处理后在体内可能仅以非常小的程度出现，但可能需要这种酶来将其潜在的有害生物学效应降至最低。
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