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不同人类细胞系在牙科树脂基修复材料生物学评价中的敏感性

Sensitivity of different human cell line in the biologic evaluation of dental resin-based restorative materials.

作者信息

Hensten-Pettersen A, Helgeland K

出版信息

Scand J Dent Res. 1981 Feb;89(1):102-7. doi: 10.1111/j.1600-0722.1981.tb01283.x.

DOI:10.1111/j.1600-0722.1981.tb01283.x
PMID:6940222
Abstract

Three different cell lines of human origin, i.e. human epithelial cells (NCTC 2544), and epithelial- and fibroblast-like cells from human gingiva, were tested for their sensitivity to three different dental resin-based restorative materials using four different assay techniques. The techniques applied were cell growth measurements by cell number, 51Cr-release, agar overlay with neutral red staining and colony formation. The ranking of the cells according to their sensitivity to the materials varied with the assay technique applied, with no cell line being consistently more sensitive than the others. It is also suggested that the type of medium applied could be important for the results obtained.

摘要

对三种不同的人类源细胞系,即人上皮细胞(NCTC 2544)以及来自人牙龈的上皮样和成纤维细胞样细胞,使用四种不同的检测技术测试了它们对三种不同的牙科树脂基修复材料的敏感性。所应用的技术包括通过细胞计数进行细胞生长测量、51Cr释放、中性红染色琼脂覆盖和集落形成。根据细胞对材料的敏感性进行的排名随所应用的检测技术而变化,没有一种细胞系始终比其他细胞系更敏感。还表明所应用的培养基类型可能对获得的结果很重要。

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