Köhler B, Pettersson B M, Bratthall D
Scand J Dent Res. 1981 Feb;89(1):19-25. doi: 10.1111/j.1600-0722.1981.tb01273.x.
Plaque samples from 10 different tooth surfaces of 10 schoolchildren with varied caries experience (DFS 10-33) were collected five times during 2.5 years. The samples were examined with an immunofluorescent technique for identification and enumeration of Streptococcus mutans serotypes c/e/f and d/g. At each sampling occasion the children were scored for caries. A stimulated saliva sample was also collected and the number of S. mutans per ml saliva was determined. The saliva level of S. mutans was shown to reflect the prevalence and proportion of this microorganism on the selected surfaces. Five surfaces carried S. mutans at each sampling. Four of these surfaces showed progressive cries S. mutans infection was also found to precede the development of incipient caries on four surfaces. Eighty percent of the surfaces that stayed sound were only transiently carriers of S. mutans in mainly very low numbers. Serotype c/e/f dominated in prevalence and proportion on the surfaces with a history of caries during the study.
在2.5年的时间里，对10名患龋经历各异（DFS为10 - 33）的学童的10个不同牙齿表面的菌斑样本进行了5次采集。采用免疫荧光技术对样本进行检测，以鉴定和计数变形链球菌血清型c/e/f和d/g。在每次采样时，对儿童的龋齿情况进行评分。同时采集一份刺激唾液样本，测定每毫升唾液中变形链球菌的数量。结果表明，唾液中变形链球菌的水平反映了该微生物在所选牙齿表面的流行情况和比例。每次采样时，有5个表面带有变形链球菌。其中4个表面出现了渐进性龋齿，在4个表面上也发现变形链球菌感染先于早期龋齿的发生。80%保持完好的表面只是变形链球菌的短暂携带者，数量通常很少。在研究期间，血清型c/e/f在有龋齿病史的表面上在流行率和比例上占主导地位。
