Sarai Naoyuki, Kagawa Wataru, Kinebuchi Takashi, Kagawa Ako, Tanaka Kozo, Miyagawa Kiyoshi, Ikawa Shukuko, Shibata Takehiko, Kurumizaka Hitoshi, Yokoyama Shigeyuki
RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
Nucleic Acids Res. 2006;34(16):4429-37. doi: 10.1093/nar/gkl562. Epub 2006 Aug 31.
The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.
同源重组过程对于减数分裂和有丝分裂细胞分裂都是不可或缺的，并且是双链断裂（DSB）修复的主要途径之一。人类Rad54B蛋白属于SWI2/SNF2蛋白家族，在同源重组中发挥作用，并且可能与Dmc1重组酶一起发挥功能，Dmc1重组酶是一种减数分裂特异性的Rad51同源物。在本研究中，我们发现Rad54B通过稳定Dmc1-单链DNA（ssDNA）复合物来增强Dmc1的DNA链交换活性。因此，Rad54B可能通过稳定核蛋白丝来刺激Dmc1介导的DNA链交换，该核蛋白丝在同源重组期间在DSB位点产生的ssDNA尾巴上形成。
