人源Rad54B蛋白对Dmc1介导的DNA链交换的刺激作用。
Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein.
作者信息
Sarai Naoyuki, Kagawa Wataru, Kinebuchi Takashi, Kagawa Ako, Tanaka Kozo, Miyagawa Kiyoshi, Ikawa Shukuko, Shibata Takehiko, Kurumizaka Hitoshi, Yokoyama Shigeyuki
机构信息
RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
出版信息
Nucleic Acids Res. 2006;34(16):4429-37. doi: 10.1093/nar/gkl562. Epub 2006 Aug 31.
The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.
同源重组过程对于减数分裂和有丝分裂细胞分裂都是不可或缺的，并且是双链断裂（DSB）修复的主要途径之一。人类Rad54B蛋白属于SWI2/SNF2蛋白家族，在同源重组中发挥作用，并且可能与Dmc1重组酶一起发挥功能，Dmc1重组酶是一种减数分裂特异性的Rad51同源物。在本研究中，我们发现Rad54B通过稳定Dmc1-单链DNA（ssDNA）复合物来增强Dmc1的DNA链交换活性。因此，Rad54B可能通过稳定核蛋白丝来刺激Dmc1介导的DNA链交换，该核蛋白丝在同源重组期间在DSB位点产生的ssDNA尾巴上形成。
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