温和噬菌体SH10介导的吸水链霉菌中的转导作用。

Transduction in Streptomyces hygroscopicus mediated by the temperate bacteriophage SH10.

作者信息

Süss F, Klaus S

出版信息

Mol Gen Genet. 1981;181(4):552-5. doi: 10.1007/BF00428752.

Abstract

The temperate actinophage SH10 mediates generalized transduction in Streptomyces hygroscopicus at low frequency. The efficiency of transduction depends on the average phage input, age of outgrowing spores of the recipient and on the selective marker. The highest EOT was found for the auxotrophic mutants 21(phe-) and 5(try-) (4.2 x 10(-6) and 2.7 x 10(-6), respectively). Transduction of the thermosensitive mutant NG14-216 ts 35 was two orders of magnitude lower (2.5 x 10(-8)). The transductant colonies segregated into stable and unstable clones. Stable transductants were never found to be lysogenic for phage SH10.

摘要

温和型肌尾噬菌体SH10在低频率下介导吸水链霉菌的普遍性转导。转导效率取决于平均噬菌体输入量、受体生长出的孢子的年龄以及选择标记。对于营养缺陷型突变体21（phe-）和5（try-），发现其最高转导效率（EOT）分别为4.2×10⁻⁶和2.7×10⁻⁶。温度敏感型突变体NG14 - 216 ts 35的转导效率低两个数量级（2.5×10⁻⁸）。转导子菌落可分为稳定和不稳定克隆。从未发现稳定转导子对噬菌体SH10具有溶原性。