链霉菌温和噬菌体R4粘性末端位点（cos）的界定
Delimitation of cohesive ends site (cos) of Streptomyces temperate bacteriophage R4.
作者信息
Mitsui H, Takahashi H
机构信息
Institute of Applied Microbiology, University of Tokyo.
出版信息
Biochem Biophys Res Commun. 1992 Aug 14;186(3):1663-8. doi: 10.1016/s0006-291x(05)81599-2.
The cohesive ends site (cos) of actinophage R4 was delimitated by assaying the in vivo packaging activity of cosmid derivatives in Streptomyces lividans. A region of 66 bp from -30 to +36 from the center of cohesive ends was required for the basal level of packaging activity. Two additional regions outside the basal sequences from -39 to -31 and from +37 to +97 were necessary for the high level of activity, defined as the accessory sequences. Direct- or inverted-repeat sequences were found within the delimitated region, which might be involved in the recognition by the terminase of actinophage R4.
通过检测粘粒衍生物在变铅青链霉菌中的体内包装活性，确定了肌动噬菌体R4的粘性末端位点（cos）。包装活性的基础水平需要一个从粘性末端中心-30到+36的66bp区域。从-39到-31以及从+37到+97的基础序列之外的另外两个区域对于高水平的活性是必需的，这两个区域被定义为辅助序列。在划定区域内发现了正向或反向重复序列，这可能参与了肌动噬菌体R4末端酶的识别。
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