Nikodem V M, Trus B L, Rall J E
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4411-5. doi: 10.1073/pnas.78.7.4411.
The composition of nucleoproteins in normal rat liver, in livers from thyroidectomized animals, and in thyroidectomized animals treated with 3,3',5-triiodo-L-thyronine was examined by two-dimensional gel electrophoresis. A computer program was designed to measure the relative concentrations of each protein. Approximately 500 protein subunits could be distinguished. Of these, 102 either disappeared or were markedly decreased after thyroidectomy. Shortly after administration of the thyronine (6 hr), 13 proteins reappeared and, by 24 hr, 67 additional proteins had reappeared. These changes in protein concentrations are both the greatest in number and have the largest reported as an effect of thyroid hormone. Twenty-four hr after triiodothyronine administration, some 22 proteins seen in normal liver could not be found. Two new proteins appeared in thyroidectomized rat liver nuclei. We conclude that a major and rapid effect of thyroid hormone is exerted on hepatic nucleoproteins.
通过二维凝胶电泳对正常大鼠肝脏、甲状腺切除动物的肝脏以及用3,3',5-三碘-L-甲状腺原氨酸治疗的甲状腺切除动物肝脏中的核蛋白组成进行了检测。设计了一个计算机程序来测量每种蛋白质的相对浓度。大约可以区分出500个蛋白质亚基。其中，102个在甲状腺切除后消失或显著减少。给予甲状腺素后不久（6小时），13种蛋白质重新出现，到24小时时，又有67种蛋白质重新出现。这些蛋白质浓度的变化在数量上是最大的，并且作为甲状腺激素的作用被报道的幅度也是最大的。给予三碘甲状腺原氨酸24小时后，在正常肝脏中可见的约22种蛋白质无法找到。在甲状腺切除大鼠肝脏细胞核中出现了两种新蛋白质。我们得出结论，甲状腺激素对肝脏核蛋白有主要且快速的作用。
