Yamaguchi H, Hirasawa K, Tanaka T, Shioiri T, Matsue I
J Periodontol. 1981 Oct;52(10):630-8. doi: 10.1902/jop.1981.52.10.630.
The purpose of this study was to determine the effect of chlorhexidine digluconate mouthrinses on the development of dental plaque in man. The dental plaques were formed on strips of triacetylcellulose film (TAC), which were attached to the lingual surfaces of the mandibular incisors by silk suture thread on 20 human volunteers. Differences in the degree of plaque formation on TAC films after 2, 4 and 24 hours were observed in placebo vs. chlorhexidine treated samples using both scanning and transmission electron microscopy. It was found that (1) solutions containing chlorhexidine digluconate and lactic aluminum have an inhibitory effect of the development of dental plaque on the TAC film as evaluated by electron microscopy, (2) the TAC film was a convenient model for the study of early dental plaque formation by electron microscopy, (3) the three types of pellicle reported by Tryggve Lie (1977) were also observed, (4) the method enabled evaluation of the bacterial accumulation of TAC films. After 2 hours, there was no visible difference in the bacterial accumulations on placebo or chlorhexidine treated films. After 4 and 24 hours considerable differences were observed.
本研究的目的是确定葡萄糖酸氯己定漱口水对人类牙菌斑形成的影响。牙菌斑在三醋酸纤维素薄膜（TAC）条上形成，通过丝线将TAC条固定在20名人类志愿者下颌切牙的舌面。使用扫描电子显微镜和透射电子显微镜观察安慰剂组与氯己定治疗组样本在2小时、4小时和24小时后TAC膜上菌斑形成程度的差异。结果发现：（1）通过电子显微镜评估，含葡萄糖酸氯己定和乳酸铝的溶液对TAC膜上牙菌斑的形成有抑制作用；（2）TAC膜是通过电子显微镜研究早期牙菌斑形成的便捷模型；（3）还观察到了Tryggve Lie（1977年）报道的三种类型的牙菌斑膜；（4）该方法能够评估TAC膜上细菌的聚集情况。2小时后，安慰剂组或氯己定治疗组薄膜上的细菌聚集情况没有明显差异。4小时和24小时后观察到了显著差异。
