Ugurbil K, Guernsey D L, Brown T R, Glynn P, Tobkes N, Edelman I S
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4843-7. doi: 10.1073/pnas.78.8.4843.
31P NMR spectra of "normal" and x-ray-transformed anchorage-dependent mouse embryo fibroblasts (C3H/10T 1/2) at 145.7 MHz and 37 degrees C were obtained by using a solenoid coil probe with a sensitivity approximately 2.6 times higher than that of the standard Helmholtz coil probe. The cells were grown as monolayers on the surface of microcarrier beads. Results show that these cells have low levels of NMR-detectable ADP, are highly impermeable to protons, are capable of maintaining high ATP levels in the absence of respiration, and, when ATP generation is blocked, ultimately hydrolyze their existing ATP to a nonphosphorylated product and Pi. Only small differences were observed between the spectra of "normal" and transformed cells. However, the latter accumulated 2-deoxyglucose 6-phosphate at a faster rate.
使用灵敏度比标准亥姆霍兹线圈探头高约2.6倍的螺线管线圈探头，在145.7兆赫和37摄氏度下获得了“正常”和经X射线转化的贴壁依赖性小鼠胚胎成纤维细胞（C3H/10T 1/2）的31P核磁共振谱。细胞在微载体珠表面以单层形式生长。结果表明，这些细胞的核磁共振可检测ADP水平较低，对质子高度不可渗透，在无呼吸的情况下能够维持高ATP水平，并且当ATP生成受阻时，最终会将其现有的ATP水解为非磷酸化产物和无机磷酸。在“正常”细胞和转化细胞的谱图之间仅观察到微小差异。然而，后者积累6-磷酸-2-脱氧葡萄糖的速度更快。
