McLaughlin A C, Takeda H, Chance B
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5445-9. doi: 10.1073/pnas.76.11.5445.
31P NMR was used to continuously monitor ATP and inorganic phosphate levels in perfused mouse liver. Under "optimal" conditions, the time resolution of the technique was approximately 1 min. In the absence of any metabolic perturbations the ATP level remained constant for at least 2 hr and decreased by only approximately 20% in 18 hr. Both ATP and inorganic phosphate levels responded to alterations in the oxygen supply to the liver. The half-time for this response was approximately 1 min, and the response to short periods of hypoxia or ischemia was partially reversible. The addition of insulin caused only a minor decrease in the ATP level but significantly decreased the rate of response of ATP and phosphate levels to hypoxia and ischemia.
采用31P核磁共振技术连续监测灌注小鼠肝脏中的ATP和无机磷酸盐水平。在“最佳”条件下，该技术的时间分辨率约为1分钟。在没有任何代谢干扰的情况下，ATP水平至少在2小时内保持恒定，18小时内仅下降约20%。ATP和无机磷酸盐水平均对肝脏氧气供应的变化做出反应。这种反应的半衰期约为1分钟，对短期缺氧或缺血的反应部分可逆。添加胰岛素仅使ATP水平略有下降，但显著降低了ATP和磷酸盐水平对缺氧和缺血的反应速率。
