Pfisterer J, Buetow D E
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4917-21. doi: 10.1073/pnas.78.8.4917.
We have isolated the translation system from yeast mitochondria and have reconstructed it in vitro. This submitochondrial system, composed of mitochondrial ribosomes, tRNA, pH 5 fraction and mRNA, is maximally active at 10 mM Mg2+ and 100 mM KCl or NH4Cl. NH4+ is more stimulatory than K+. Added Escherichia coli tRNA gives less than half the activity obtained with added mitochondrial tRNA. Activity is enhanced with protease inhibitors but not with Ca2+, spermine, or spermidine. In contrast to heterologous translation systems, the present system produces products with molecular weights similar to those of products synthesized by yeast mitochondria in vivo and by intact yeast mitochondria in vitro. The results support the idea that the unique coding features of the mitochondrial genome require a unique translation system for accurate translation of mitochondrial mRNAs.
我们从酵母线粒体中分离出翻译系统，并在体外进行了重建。这个亚线粒体系统由线粒体核糖体、tRNA、pH 5组分和mRNA组成，在10 mM Mg2+和100 mM KCl或NH4Cl条件下活性最高。NH4+的刺激作用比K+更强。添加大肠杆菌tRNA所产生的活性不到添加线粒体tRNA所获活性的一半。蛋白酶抑制剂可增强活性，但Ca2+、精胺或亚精胺则无此作用。与异源翻译系统不同，本系统产生的产物分子量与酵母线粒体在体内以及完整酵母线粒体在体外合成的产物相似。这些结果支持了这样一种观点，即线粒体基因组独特的编码特征需要一个独特的翻译系统来准确翻译线粒体mRNA。
