Lawrence P A
J Embryol Exp Morphol. 1981 Aug;64:321-32.
The mitochondrial enzyme, succinate dehydrogenase, can be localized by specific histochemical stains. A naturally occurring variant which gives heat-labile enzyme is used to map a gene responsible for succinate dehydrogenase. New alleles at the sdh locus are produced by mutagenesis and most of these are found to be homozygous lethal to flies. However, clones of cells which are homozygous for the new alleles can be produced by mitotic recombination, and are found to develop normally. If the chromosome arm bearing an sdh allele also carried brown, clones can be found in the eye. At the border of the clone the brown and sdh phenotype coincide showing that the sdh phenotype is expressed as a cell autonomous marker. Clones of sdh have been observed in the adult epidermis, the muscles, the gut, the heart and oenocytes where they display a clear phenotype. This new marker may be useful in studying the development of the internal organs of Drosophila.
线粒体酶琥珀酸脱氢酶可通过特定的组织化学染色进行定位。一种产生热不稳定酶的天然变体被用于定位负责琥珀酸脱氢酶的基因。通过诱变产生了sdh位点的新等位基因，其中大多数被发现对果蝇是纯合致死的。然而，通过有丝分裂重组可以产生新等位基因纯合的细胞克隆，并且发现它们发育正常。如果携带sdh等位基因的染色体臂也携带棕色基因，那么在眼睛中就能发现克隆。在克隆的边界处，棕色和sdh表型一致，这表明sdh表型作为一种细胞自主标记物被表达。在成年表皮、肌肉、肠道、心脏和oenocytes中观察到了sdh克隆，它们表现出明显的表型。这种新的标记物可能有助于研究果蝇内脏器官的发育。
