Maeda T, Taguchi H, Nomura Y, Ikeda H, Iseki T, Nakamura M, Kawahara H
Shika Rikogaku Zasshi. 1981 Apr;22(59):162-7.
In order to examine eligibility of supersonic vibration energy for investigating cell-material contract, L-strain fibroblasts were cultivated on glass surface and exposed to supersonic vibration. The cells adhered to the glass surface were detached by supersonic vibration and both of adhered cells to the glass surface and detached cells after the supersonic exposure were cultivated for one week. And the cell activity of the detached cells was estimated with cell multiplication by cell nuclei counting and their morphological changes. As a result, use of supersonic vibration energy was found effective in expressing degrees of cell adhesiveness to glass or biomaterials. The most appropriate vibration was decided at 485 KHz in frequency, 5 V in secondary voltage and approximately 30 seconds. It is revealed that the results may be effective in the analysis of cell-biomaterial contact.
为了研究超声振动能量在研究细胞与材料相互作用方面的适用性，将L-应变成纤维细胞培养在玻璃表面并暴露于超声振动。通过超声振动使粘附在玻璃表面的细胞脱落，将超声暴露后粘附在玻璃表面的细胞和脱落的细胞都培养一周。通过细胞核计数的细胞增殖及其形态变化来评估脱落细胞的细胞活性。结果发现，使用超声振动能量可有效表达细胞对玻璃或生物材料的粘附程度。最适宜的振动频率为485千赫兹，二次电压为5伏，持续时间约30秒。结果表明，这些结果可能对细胞-生物材料接触的分析有效。
