[Effects of electrical stimulus upon L-strain cells. (Part 2)--Response of the cells to alternative current prior to cultivation (author's transl)].

In order to examine the effects of voltage, current time and frequency of the alternative current on cell multiplication and morphology during initial stage of cell cultivation, alternative currents of varying intensity were applied to the L-strain cells in the tissue culture medium, i.e. YLH solution supplemented with 5% bovine serum which was considered as an electrolyte solution. The following results were obtained: 1) The rates of cell multiplication depended on the frequency (0.01 Hz-10 KHz), when voltage and current time were kept constant. In particular, they were remarkably depressed in the low frequency range. In a certain frequency range, a maximum value of the rates of cell multiplication was recorded. It was considered that there is certainly a least inhibitive frequency range in the rates of cell multiplication. 2) The lower and the shorter the voltage and current time were, the lower were the frequency level at the threshold of marked inhibition of the rates of cell multiplication brought. 3) An arbitrary equation for the relationship between the rates of cell multiplication and frequency was determined: The rates of cell multiplication upon cell multiplication were proportional to (A + log F), where A was a constant value and F was the applied frequency. According to the equation above, the depression upon cell multiplication was calculated, when the values of voltage and current time were given. 4) It was found that the results of the rates of cell multiplication were parallel to the results of the morphological observations. It was presumed that the main factor governing cell multiplication during application of the alternative current was due to change of the compositions of medium which was electrolyzed.