Maeda T, Kawahara H, Nakamura M, Mise T, Koda Y, Isomura S, Yokota J
Shika Rikogaku Zasshi. 1977 Oct;18(44):237-53.
In order to examine the effects of voltage, current time and frequency of the alternative current on cell multiplication and morphology during initial stage of cell cultivation, alternative currents of varying intensity were applied to the L-strain cells in the tissue culture medium, i.e. YLH solution supplemented with 5% bovine serum which was considered as an electrolyte solution. The following results were obtained: 1) The rates of cell multiplication depended on the frequency (0.01 Hz-10 KHz), when voltage and current time were kept constant. In particular, they were remarkably depressed in the low frequency range. In a certain frequency range, a maximum value of the rates of cell multiplication was recorded. It was considered that there is certainly a least inhibitive frequency range in the rates of cell multiplication. 2) The lower and the shorter the voltage and current time were, the lower were the frequency level at the threshold of marked inhibition of the rates of cell multiplication brought. 3) An arbitrary equation for the relationship between the rates of cell multiplication and frequency was determined: The rates of cell multiplication upon cell multiplication were proportional to (A + log F), where A was a constant value and F was the applied frequency. According to the equation above, the depression upon cell multiplication was calculated, when the values of voltage and current time were given. 4) It was found that the results of the rates of cell multiplication were parallel to the results of the morphological observations. It was presumed that the main factor governing cell multiplication during application of the alternative current was due to change of the compositions of medium which was electrolyzed.
为了研究在细胞培养初期，电压、电流时间和交流电频率对细胞增殖及形态的影响，将不同强度的交流电施加于组织培养基中的L - 株细胞，即添加5%牛血清的YLH溶液，该溶液被视为电解质溶液。得到以下结果：1）当电压和电流时间保持恒定时，细胞增殖速率取决于频率（0.01Hz - 10KHz）。特别是在低频范围内，细胞增殖速率显著降低。在一定频率范围内，记录到细胞增殖速率的最大值。据认为，细胞增殖速率中肯定存在一个最小抑制频率范围。2）电压和电流时间越低越短，导致细胞增殖速率明显受抑制的频率阈值水平就越低。3）确定了细胞增殖速率与频率之间关系的一个任意方程：细胞增殖时的细胞增殖速率与（A + log F）成正比，其中A为常数，F为施加的频率。根据上述方程，当给出电压和电流时间的值时，可计算出对细胞增殖的抑制作用。4）发现细胞增殖速率的结果与形态学观察结果平行。据推测，在施加交流电期间，控制细胞增殖的主要因素是由于被电解的培养基成分发生了变化。
