Tran Van P, Mailland M L
J Biol Buccale. 1981 Dec;9(4):385-400.
This histological study was designed to observe the early effects of occlusal hypofunction on periodontal tissues. Eighteen Wistar rats were used. Hypofunction was induced by extracting the right maxillary molars. Histological observations were reported on the right lower jaws which were embedded in methyl-metacrylate, sectioned at 4 micrometer without decalcification and stained with toluidine blue. The periodontal ligament disorganization began after 2 days and continued until 16 days. The outstanding observation was a considerable increase in bone formation, which was responsible for ligament narrowing as no change in cementoblastic activity was observed. At the top of the interradicular septa, the osteoblastic proliferation began at 24 hours, and bone mineralization increased until 4 days creating osteophytic bone, and then decreased. On the modelling sides of the sockets, a great amount of osteoid tissue deposited by osteoblasts set around large vessels was present at 4 days, and mineralization extended between 4 and 8 days. On the remodelling sides, bone formation extended at 4 days and osteoclastic resorption fell of except along oblique roots sockets; then the remodelling sides returned to nearly normal aspect at 16 days. The biological significance and clinical implications of these findings are discussed.
本组织学研究旨在观察咬合功能减退对牙周组织的早期影响。使用了18只Wistar大鼠。通过拔除右上颌磨牙诱导功能减退。对嵌入甲基丙烯酸甲酯中的右下颌骨进行组织学观察，在不脱钙的情况下切成4微米厚的切片，并用甲苯胺蓝染色。牙周膜紊乱在2天后开始，并持续至16天。显著的观察结果是骨形成显著增加，这是导致韧带变窄的原因，因为未观察到成牙骨质细胞活性的变化。在根间隔顶部，成骨细胞增殖在24小时开始，骨矿化增加直至4天形成骨赘性骨，然后减少。在牙槽窝的塑形侧，4天时在大血管周围有成骨细胞沉积的大量类骨组织，矿化在4至8天之间扩展。在改建侧，骨形成在4天时扩展，破骨细胞吸收减少，但沿斜根牙槽窝处除外；然后改建侧在16天时恢复到几乎正常的外观。讨论了这些发现的生物学意义和临床意义。
