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Isolation, purification, and characterization of mouse placental lactogen.

作者信息

Colosi P, Marr G, Lopez J, Haro L, Ogren L, Talamantes F

出版信息

Proc Natl Acad Sci U S A. 1982 Feb;79(3):771-5. doi: 10.1073/pnas.79.3.771.

DOI:10.1073/pnas.79.3.771
PMID:6950428
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345834/
Abstract

Mouse placental lactogen was purified 1840-fold from BALB/c placentae from days 14-18 of gestation with an overall yield of 29%. The purification procedure included alkaline homogenization and extraction, ammonium sulfate precipitation, hydrophobic interaction chromatography on Phenyl-Sepharose, ion-exchange chromatography on CM- and DEAE-cellulose, and gel exclusion chromatography on Sephadex G-100. On 10% alkaline polyacrylamide gels, mouse placental lactogen had an Rf of 0.19. Electrophoresis in gels containing NaDodSO4 showed a single band with a mobility corresponding to a Mr of 23,000 +/- 1000. The isoelectric point, determined by isoelectric focusing in 8 M urea/5% 2-mercaptoethanol, was 7.1. When tested in the pigeon crop sac assay, 10 micrograms of mouse placental lactogen produced stimulation comparable with that evoked by 10 micrograms of ovine prolactin. In the rabbit mammary gland radioreceptor assay, mouse placental lactogen was 150% more potent than ovine prolactin in displacing 125I-labeled ovine prolactin from rabbit mammary gland membranes. Iodinated purified mouse placental lactogen could be displaced from rabbit mammary gland membranes by mouse placental lactogen, mouse prolactin, and ovine prolactin. Ovine prolactin was 45% as avid as mouse placental lactogen is displacing 125I-labeled mouse placental lactogen from rabbit mammary gland membranes. Mouse placental lactogen did not crossreact with antisera to mouse prolactin or mouse growth hormone in a radioimmunoassay.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/fb13d8cc8565/pnas00442-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/68d1c35364a8/pnas00442-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/7c4646595ef6/pnas00442-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/7b30a447ae89/pnas00442-0061-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/d317e713510d/pnas00442-0061-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/fb13d8cc8565/pnas00442-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/68d1c35364a8/pnas00442-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/7c4646595ef6/pnas00442-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/7b30a447ae89/pnas00442-0061-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/d317e713510d/pnas00442-0061-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2470/345834/fb13d8cc8565/pnas00442-0062-a.jpg

相似文献

1
Isolation, purification, and characterization of mouse placental lactogen.
Proc Natl Acad Sci U S A. 1982 Feb;79(3):771-5. doi: 10.1073/pnas.79.3.771.
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The purification and characterization of rabbit placental lactogen.兔胎盘催乳素的纯化与特性分析
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3

本文引用的文献

1
STUDIES OF A HUMAN AND SIMIAN PLACENTAL HORMONE WITH GROWTH HORMONE-LIKE AND PROLACTIN-LIKE ACTIVITIES.具有生长激素样和催乳素样活性的人类及猿猴胎盘激素的研究。
J Clin Endocrinol Metab. 1964 Jan;24:80-100. doi: 10.1210/jcem-24-1-80.
2
Isolation and partial characterization of secreted hamster pituitary prolactin.
Endocrinology. 1981 Mar;108(3):850-4. doi: 10.1210/endo-108-3-850.
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Homologous radioimmunoassay for secreted mouse prolactin.
Life Sci. 1981 Jan 12;28(2):203-11. doi: 10.1016/0024-3205(81)90554-3.
4
Interleukin 6 inhibits mouse placental lactogen II but not mouse placental lactogen I secretion in vitro.
白细胞介素6在体外抑制小鼠胎盘催乳素II的分泌，但不抑制小鼠胎盘催乳素I的分泌。
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11905-9. doi: 10.1073/pnas.90.24.11905.
4
Opposite effects of transforming growth factor alpha and epidermal growth factor on mouse placental lactogen I secretion.转化生长因子α和表皮生长因子对小鼠胎盘催乳素I分泌的相反作用。
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2830-4. doi: 10.1073/pnas.92.7.2830.
5
Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family.编码催乳素-生长激素家族成员的与生长相关的信使核糖核酸的核苷酸序列。
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4255-9. doi: 10.1073/pnas.81.14.4255.
6
A new member of the prolactin-growth hormone gene family expressed in mouse placenta.一种在小鼠胎盘中表达的催乳素-生长激素基因家族的新成员。
EMBO J. 1985 Jun;4(6):1419-23. doi: 10.1002/j.1460-2075.1985.tb03796.x.
7
Identification of proliferin mRNA and protein in mouse placenta.小鼠胎盘中增殖蛋白mRNA和蛋白的鉴定。
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4356-9. doi: 10.1073/pnas.82.13.4356.
8
Molecular cloning of a prolactin-related mRNA expressed in bovine placenta.在牛胎盘中表达的一种催乳素相关信使核糖核酸的分子克隆
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5650-4. doi: 10.1073/pnas.84.16.5650.
9
Molecular cloning of mouse placental lactogen cDNA.小鼠胎盘催乳素cDNA的分子克隆
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8496-500. doi: 10.1073/pnas.83.22.8496.
10
Placental-specific expression from the mouse placental lactogen II gene promoter.
Proc Natl Acad Sci U S A. 1992 May 1;89(9):3864-8. doi: 10.1073/pnas.89.9.3864.
Two forms of rat placental lactogen revealed by radioimmunoassay.放射免疫分析法显示的两种大鼠胎盘催乳素形式。
Endocrinology. 1981 Jun;108(6):2388-90. doi: 10.1210/endo-108-6-2388.
5
Serum placental lactogen in mice in relation to day of gestation and number of conceptuses.
Biol Reprod. 1981 May;24(4):846-51. doi: 10.1095/biolreprod24.4.846.
6
The lactogenic response of mouse mammary explants to mose prolactin and growth hormone.
Endocr Res Commun. 1980;7(4):269-78. doi: 10.3109/07435808009065978.
7
Phylogenetic distribution, regulation of secretion, and prolactin-like effects of placental lactogens.胎盘催乳素的系统发育分布、分泌调节及催乳素样作用
Fed Proc. 1980 Jun;39(8):2582-7.
8
Bio-assay of prolactin. Analysis of the pigeon crop-sac response to local prolactin injection by an objective and quantitative method.催乳素的生物测定。用客观定量方法分析鸽子嗉囊对局部注射催乳素的反应。
Endocrinology. 1967 Apr;80(4):641-55. doi: 10.1210/endo-80-4-641.
9
Kinetic study of the deamidation of growth hormone and prolactin.生长激素和催乳素脱酰胺作用的动力学研究
Biochim Biophys Acta. 1970 Sep 29;214(3):498-508. doi: 10.1016/0005-2795(70)90310-7.
10
Quantitative participation of placental mammotropic hormones in mammary development during pregnancy of mice.胎盘促乳激素在小鼠孕期乳腺发育中的定量参与情况。
Endocrinol Jpn. 1971 Dec;18(6):507-10. doi: 10.1507/endocrj1954.18.507.