在A、C、G或R显带程序后对染色体核仁组织区进行银染。
Ag-staining of nucleolus organizer regions of chromosomes after A-,C-, G-, or R-banding procedures.
作者信息
Tantravahi R, Miller D A, Miller O J
出版信息
Cytogenet Cell Genet. 1977;18(6):364-9. doi: 10.1159/000130782.
PMID:69519
Abstract
Metaphase chromosome preparations were made from leukocyte cultures of normal individuals. The cells were fixed in methanol:acetic acid (3:1 v/v), then dropped on cold, wet slides which were air-dried before storage at 4 degrees C. The slides were stained to identify the chromosomes by one of the following procedures: (1) Quinacrine. Slides were stained for 10 min in quinacrine mustard solution, rinsed in running tap water for 2 min, and mounted in Tris-maleat buffer, pH 5.6.
摘要
中期染色体标本取自正常个体的白细胞培养物。细胞用甲醇：乙酸（3:1 v/v）固定，然后滴在冰冷、湿润的载玻片上，空气干燥后于4℃保存。载玻片通过以下方法之一进行染色以识别染色体：（1）喹吖因。载玻片在喹吖因芥子溶液中染色10分钟，在流动自来水中冲洗2分钟，然后用pH 5.6的Tris-马来酸盐缓冲液封片。
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