Miller D A, Tantravahi R, Dev V G, Miller O J
Am J Hum Genet. 1977 Sep;29(5):490-502.
Methaphase chromosomes from karyotypically normal adult humans (three males, six females) and one male with a 13p - chromosome were stained by quinacrine and then by the Ag-AS silver staining method to reveal nucleolus organizer regions (NORs). Each person had a characteristic number of Ag-stained chromosomes per cell, always fewer than 10. Determination of the mean Ag-size of each chromosome showed that each of the 10 individuals had a unique distribution of Ag-stain. Within each individual, there was some variation from cell to cell in the number of acrocentric chromosomes that were Ag-stained; this was not random, and the same chromosomes (those that had at most a small amount of Ag-stain) tended to be unstained in every cell. Satellite associations were scored on the same cells. Chromosomes that had no Ag-stain were involved in satellite association less than 20% as often as those that had some Ag-stain. Chromosomes that had a small amount of Ag-stain were involved in association about 50% as often as those that had a large amount of stain. Regression analysis of the 50 (of a total of 100) acrocentric chromosomes which could be individually identified by quinacrine markers showed that the frequency with which a chromosome was involved in satellite association was strongly correlated with the amount of Ag-stained material in the NOR.
对核型正常的成年人类（三名男性、六名女性）以及一名患有13号染色体短臂缺失的男性的中期染色体先用喹吖因染色，然后采用银染-抗银染色法来显示核仁组织区（NORs）。每个人每个细胞中银染染色体的数量都具有特征性，总是少于10条。对每条染色体的平均银染大小进行测定表明，这10个人中的每个人都有独特的银染分布。在每个个体内部，银染的近端着丝粒染色体数量在细胞之间存在一些差异；这并非随机现象，并且相同的染色体（那些银染量最多只有少量的染色体）在每个细胞中往往都不被染色。对相同的细胞进行卫星体联会计数。没有银染的染色体参与卫星体联会的频率不到有一些银染的染色体的20%。有少量银染的染色体参与联会的频率约为有大量银染的染色体的50%。对总共100条近端着丝粒染色体中能用喹吖因标记单独识别的50条染色体进行回归分析表明，一条染色体参与卫星体联会的频率与NOR中银染物质的量密切相关。
