豆状中间丝：生物合成及其与质膜的相互作用

Ramaekers F C, Dunia I, Dodemont H J, Benedetti E L, Bloemendal H

Proc Natl Acad Sci U S A. 1982 May;79(10):3208-12. doi: 10.1073/pnas.79.10.3208.

Electron microscopical features of the lens fiber plasma membrane-cytoskeleton complex are suggestive of an intimate association between the intermediate-sized filaments (IF) and the lipid bilayer. Biochemical analysis of this complex reveals the occurrence of an appreciable amount of vimentin as a protein subunit of lenticular IF. Additional evidence for association between IF and membranes is provided by the observation that newly synthesized vimentin is associated with plasma membranes added to a reticulocyte lysate programmed with lens polyribosomes. Concomitantly alpha-crystallin polypeptide chains (alpha A2) are also found associated with the plasma membrane together with a hitherto unidentified 47-kilodalton protein. Once associated with the lipid bilayer, the vimentin polypeptide resists urea treatment, suggesting that it has become an integral constituent associated with part of the membrane.

晶状体纤维质膜 - 细胞骨架复合体的电子显微镜特征表明，中间丝（IF）与脂质双层之间存在密切关联。对该复合体的生化分析显示，有相当数量的波形蛋白作为晶状体IF的蛋白质亚基存在。IF与膜之间存在关联的额外证据来自以下观察结果：新合成的波形蛋白与添加到用晶状体多核糖体编程的网织红细胞裂解物中的质膜相关联。同时，α - 晶状体蛋白多肽链（αA2）也与质膜以及一种迄今未鉴定的47千道尔顿蛋白质相关联。一旦与脂质双层结合，波形蛋白多肽就能抵抗尿素处理，这表明它已成为与膜的一部分相关的整合成分。