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猪心脂酰胺脱氢酶与人红细胞谷胱甘肽还原酶之间的氨基酸序列同源性。

Amino acid sequence homology between pig heart lipoamide dehydrogenase and human erythrocyte glutathione reductase.

作者信息

Williams C H, Arscott L D, Schulz G E

出版信息

Proc Natl Acad Sci U S A. 1982 Apr;79(7):2199-201. doi: 10.1073/pnas.79.7.2199.

DOI:10.1073/pnas.79.7.2199
PMID:6954534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346158/
Abstract

Extensive amino acid sequence homology has been found between nine tryptic peptides of pig heart lipoamide dehydrogenase (NADH:lipoamide oxidoreductase, EC 1.6.4.3] and the sequence of human erythrocyte glutathione reductase [NAD(P)H:glutathione oxidoreductase, EC 1.6.4.2]. The average homology is 40%. Six lipoamide dehydrogenase peptides are homologous with segments of the two parts of the FAD domain of glutathione reductase, one with the NADPH domain, and two with the interface domain. Thus, the homology extends throughout the molecule.

摘要

已发现猪心脂酰胺脱氢酶（NADH：脂酰胺氧化还原酶，EC 1.6.4.3）的九条胰蛋白酶肽段与人类红细胞谷胱甘肽还原酶（NAD(P)H：谷胱甘肽氧化还原酶，EC 1.6.4.2）的序列之间存在广泛的氨基酸序列同源性。平均同源性为40%。六条脂酰胺脱氢酶肽段与谷胱甘肽还原酶FAD结构域两部分的片段同源，一条与NADPH结构域同源，两条与界面结构域同源。因此，同源性贯穿整个分子。

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Proc Natl Acad Sci U S A. 1982 Apr;79(7):2199-201. doi: 10.1073/pnas.79.7.2199.
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本文引用的文献

1
Active-site probes of flavoproteins.黄素蛋白的活性位点探针
Biochem Soc Trans. 1980 Jun;8(3):246-57. doi: 10.1042/bst0080246.
2
Rapid separation of amino acid phenylthiohydantoins by isocratic high-performance liquid chromatography.通过等度高效液相色谱法快速分离氨基酸苯硫代乙内酰脲
Anal Biochem. 1981 Feb;111(1):27-32. doi: 10.1016/0003-2697(81)90223-2.
3
Glutathione reductase from human erythrocytes. The sequences of the NADPH domain and of the interface domain.来自人红细胞的谷胱甘肽还原酶。NADPH结构域和界面结构域的序列。
Eur J Biochem. 1982 Jan;121(2):259-67. doi: 10.1111/j.1432-1033.1982.tb05780.x.
4
Glutathione reductase from human erythrocytes: amino-acid sequence of the structurally known FAD-binding domain.来自人红细胞的谷胱甘肽还原酶：结构已知的FAD结合结构域的氨基酸序列。
Eur J Biochem. 1981 Nov;120(2):407-19. doi: 10.1111/j.1432-1033.1981.tb05718.x.
5
Glutathione reductase from yeast. Differential reactivity of the nascent thiols in two-electron reduced enzyme and properties of a monoalkylated derivative.酵母谷胱甘肽还原酶。双电子还原酶中新生硫醇的差异反应性及单烷基化衍生物的性质。
Biochemistry. 1981 Mar 17;20(6):1513-20. doi: 10.1021/bi00509a016.
6
Lipoamide dehydrogenase from pig heart. Pyridine nucleotide induced changes in monoalkylated two-electron reduced enzyme.
Biochemistry. 1981 Mar 17;20(6):1507-13. doi: 10.1021/bi00509a015.
7
Primary and tertiary structure studies of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Isolation and alignment of the CNBr peptides; interactions of the protein with flavin adenine dinucleotide.荧光假单胞菌对羟基苯甲酸羟化酶的一级结构和三级结构研究。CNBr肽段的分离与比对；该蛋白质与黄素腺嘌呤二核苷酸的相互作用。
Eur J Biochem. 1980 Dec;113(1):141-50.
8
The phylogeny of prokaryotes.原核生物的系统发育。
Science. 1980 Jul 25;209(4455):457-63. doi: 10.1126/science.6771870.
9
Comparison of the amino acid sequence of bovine alpha-lactalbumin and hens egg white lysozyme.牛α-乳白蛋白与鸡蛋清溶菌酶氨基酸序列的比较。
J Biol Chem. 1967 Aug 25;242(16):3747-9.
10
The complete amino acid sequence of bovine alpha-lactalbumin.牛α-乳白蛋白的完整氨基酸序列。
J Biol Chem. 1970 Sep 10;245(17):4570-82.