Gierek T, Lisiewicz T, Pilch J
Folia Haematol Int Mag Klin Morphol Blutforsch. 1977;104(2):208-15.
In 20 men, aged 35 to 55 years, with untreated cancer of the larynx activity of lysosomal acid phosphatase (AP), beta-glucuronidase (GR) and N-acetyl-beta-glucosaminidase was determined cytochemically in peripheral blood lymphocytes and neutrophils by means of Barka and Anderson, Hayashi et al. and Hayashi's method, respectively; the results obtained were compared with those in 20 healthy men aged 20 to 30 years. Total count of GR-positive lymphocytes was higher in the patients than in normal persons. Total counts of AP-, GR-, and GS-positive lymphocytes with not disrupted enzyme-positive lysosomal granules within the cell cytoplasm were significantly lower and total counts of cells exhibiting the disruption of lysosomal granules and the diffuse type of cytochemical reaction were significantly higher in the patients when compared with the control group. The response of neutrophils consisted of a significant elevation in numbers of AP-, and GS-positive cells; overall score of enzyme activity studied in neutrophils was not altered in the patients. The authors disucss the significance of their observations in the light of data on participation of lymphocytic and neutrophilic lysosomal apparatus in the immunological response against tumour specific antigen in patients with cancer.
对20名年龄在35至55岁之间、未经治疗的喉癌患者，分别采用巴尔卡和安德森法、林等人的方法以及林的方法，通过细胞化学方法测定外周血淋巴细胞和中性粒细胞中溶酶体酸性磷酸酶（AP）、β - 葡萄糖醛酸酶（GR）和N - 乙酰 - β - 氨基葡萄糖苷酶的活性；将所得结果与20名年龄在20至30岁的健康男性的结果进行比较。患者中GR阳性淋巴细胞的总数高于正常人。与对照组相比，患者细胞质内酶阳性溶酶体颗粒未被破坏的AP、GR和GS阳性淋巴细胞的总数显著降低，而溶酶体颗粒被破坏且呈现弥漫型细胞化学反应的细胞总数显著升高。中性粒细胞的反应表现为AP和GS阳性细胞数量显著增加；患者中性粒细胞中所研究的酶活性总分未改变。作者根据关于淋巴细胞和中性粒细胞溶酶体装置参与癌症患者针对肿瘤特异性抗原的免疫反应的数据，讨论了他们观察结果的意义。
