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外周血淋巴细胞中酸性磷酸酶、β-葡萄糖醛酸酶和β-氨基葡萄糖苷酶活性的半定量细胞化学测定

Semiquantitative cytochemical determination of acid phosphatase, beta-glucuronidase, and beta-glucosaminidase activity in peripheral blood lymphocytes.

作者信息

Lisiewicz J

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1975;102(6):625-33.

PMID:54313
Abstract

Activity of acid phosphatase, beta-glucuronidase, and beta-glucosaminidase in peripheral blood lymphocytes was determined cytochemically in 20 normal subjects, 10 male and 10 female, by the use of BARKA and ANDERSON's (1962), HAYASHI et al. (1964) and HAYASHI's (1965) methods, respectively. Results obtained were semiquantitatively according to subdivision of lymphocytes into enzyme-negative and enzyme-positive cells. Enzyme-positive lymphocytes were divided into cells with granular, mixed granular and diffuse enzymatic reaction type. In the first two types of cytochemical reaction a number of enzyme-positive lysosomal granules were counted and expressed in terms of both absolute count and percentage of circulating lymphocytes. Enzyme-positive lymphocytes represented 80.3%, 40.5% and 41.5% of the total lymphocyte count in regard to the presence of acid phosphatase, beta-glucuronidase, and beta-glucosaminidase, respectively.

摘要

采用BARKA和ANDERSON(1962年)、林等人(1964年)以及林(1965年)的方法,分别对20名正常受试者(10名男性和10名女性)外周血淋巴细胞中的酸性磷酸酶、β - 葡萄糖醛酸酶和β - 氨基葡萄糖苷酶活性进行了细胞化学测定。根据淋巴细胞分为酶阴性和酶阳性细胞的情况,对所得结果进行半定量分析。酶阳性淋巴细胞又分为具有颗粒状、混合颗粒状和弥漫性酶反应类型的细胞。在前两种细胞化学反应类型中,对一些酶阳性溶酶体颗粒进行计数,并以绝对计数和循环淋巴细胞百分比表示。就酸性磷酸酶、β - 葡萄糖醛酸酶和β - 氨基葡萄糖苷酶的存在而言,酶阳性淋巴细胞分别占淋巴细胞总数的80.3%、40.5%和41.5%。

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Semiquantitative cytochemical determination of acid phosphatase, beta-glucuronidase, and beta-glucosaminidase activity in peripheral blood lymphocytes.外周血淋巴细胞中酸性磷酸酶、β-葡萄糖醛酸酶和β-氨基葡萄糖苷酶活性的半定量细胞化学测定
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引用本文的文献

1
N-acetyl-beta-glucosaminidase of peripheral blood lymphocytes in ontogenetic development of man.人个体发育过程中外周血淋巴细胞的N-乙酰-β-葡萄糖苷酶
Blut. 1983 Apr;46(4):209-15. doi: 10.1007/BF00320640.
2
Activity of N-acetyl-beta-glucosaminidase in lymphocytes of rats exposed to mixture of nitrogen oxides and chlorine.暴露于氮氧化物和氯混合物的大鼠淋巴细胞中N-乙酰-β-葡萄糖苷酶的活性
Arch Toxicol. 1979 Dec;43(2):147-52. doi: 10.1007/BF00333622.