Gierek T, Lisiewicz J, Pilch J, Sasiadek U Y, Namyslowski G
Folia Haematol Int Mag Klin Morphol Blutforsch. 1978;105(5):640-5.
In 24 men with precancerous states of the larynx, i.e. leukoplakia, papillomas and pachydermia, the peripheral blood lymphocytes were cytochemically stained for N-acetyl-beta-glucosaminidase, beta-glucuronidase, acid phosphatase and glycogen (PAS reaction). The results were expressed in terms of the absolute counts of enzyme- (or compound-) positive cells. The serum immunoglobulin IgG, IgA and IgM levels were also determined by Mancini's method. The results obtained were compared with those in 20 healthy men aged 20 to 30 years. It was found that the patients exhibited elevated numbers of N-acetyl-beta-glucosaminidase- and beta-glucuronidase-positive lymphocytes. A characteristic feature was an increase in the absolute counts of lymphocytes with diffuse and granular-diffuse types of cytochemical reaction for all enzymes studied. The number of cells with the granular type of enzymatic reaction (intact enzyme-positive lysosomes) was significantly diminished. These cytochemical alterations were accompanied by a significant increase in the serum IgA level. These results are discussed with reference to the lymphoid system response to tissues with precancerous lesions of the larynx.
对24名患有喉癌前病变（即白斑病、乳头状瘤和厚皮病）的男性患者，采用细胞化学方法对其外周血淋巴细胞进行N - 乙酰 - β - 氨基葡萄糖苷酶、β - 葡萄糖醛酸酶、酸性磷酸酶和糖原（PAS反应）染色。结果以酶（或化合物）阳性细胞的绝对计数表示。血清免疫球蛋白IgG、IgA和IgM水平也采用曼奇尼法测定。将所得结果与20名年龄在20至30岁的健康男性的结果进行比较。发现患者中N - 乙酰 - β - 氨基葡萄糖苷酶和β - 葡萄糖醛酸酶阳性淋巴细胞数量增加。一个特征性表现是，对于所有研究的酶，具有弥漫性和颗粒 - 弥漫性细胞化学反应类型的淋巴细胞绝对计数增加。具有颗粒型酶反应（完整的酶阳性溶酶体）的细胞数量显著减少。这些细胞化学改变伴随着血清IgA水平的显著升高。结合喉癌前病变组织的淋巴系统反应对这些结果进行了讨论。
