Tanida O, Kaneshima S, Iitsuka Y, Kuda H, Kiyasu Y, Koga S
Acta Cytol. 1982 Sep-Oct;26(5):681-7.
The viability and morphologic changes of intraperitoneal free cancer cells in advanced gastric cancer patients were examined by Giemsa and enzymologic staining and by tritiated thymidine uptake. Although many free cancer cells in the pouch of Douglas showed moderate degeneration, viable, morphologically intact cells were noted, leading to the possibility of their implantation and proliferation in the peritoneum. Serosal cancer cells showed a high degree of viability. In patients undergoing gastric cancer surgery, the viability of free cancer cells was markedly decreased by a single intraoperative administration of 10 mg of mitomycin C (MMC) to the pouch of Douglas, suggesting that this may represent an effective means of preventing peritoneal dissemination.
通过吉姆萨染色、酶学染色以及氚标记胸腺嘧啶核苷摄取试验，对晚期胃癌患者腹腔游离癌细胞的活力及形态学变化进行了检测。尽管Douglas陷凹处的许多游离癌细胞呈现中度变性，但仍发现了形态完整的存活细胞，这提示它们有可能在腹膜种植并增殖。浆膜癌细胞显示出高度的活力。在接受胃癌手术的患者中，术中向Douglas陷凹单次注射10毫克丝裂霉素C（MMC）可使游离癌细胞的活力显著降低，这表明这可能是预防腹膜播散的一种有效方法。
