利用克隆的cDNA探针通过核酸杂交检测鱼类组织中的方形病毒RNA。
Detection of aquareovirus RNA in fish tissues by nucleic acid hybridization with a cloned cDNA probe.
作者信息
Subramanian K, Lupiani B, Hetrick F M, Samal S K
机构信息
College of Veterinary Medicine, University of Maryland, College Park 20742.
出版信息
J Clin Microbiol. 1993 Jun;31(6):1612-4. doi: 10.1128/jcm.31.6.1612-1614.1993.
Abstract
A nucleic acid hybridization assay was developed to rapidly detect small quantities of aquareovirus RNAs in infected cells and organs. Cloned cDNA copies were synthesized from the genomic RNA of the SBR strain of aquareovirus. By using cloned cDNA probes, aquareovirus RNAs were detected in spleen and kidney tissues of experimentally infected fish.
摘要
开发了一种核酸杂交试验，用于快速检测感染细胞和器官中的少量水生呼肠孤病毒RNA。从水生呼肠孤病毒SBR株的基因组RNA合成了克隆的cDNA拷贝。通过使用克隆的cDNA探针，在实验感染鱼的脾脏和肾脏组织中检测到了水生呼肠孤病毒RNA。
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