Cohen-Solal M M, Authier B, deRiel J K, Murnane M J, Forget B G
DNA. 1982;1(4):355-63. doi: 10.1089/dna.1982.1.355.
Clones of human embryonic alpha-like zeta-globin cDNA were isolated, by detection using cross-hybridization to human alpha-globin cDNA probes, from a cDNA library derived from the mRNA of the human erythroleukemia cell line K562. Nucleotide sequence analysis of these cDNA clones revealed a coding sequence that corresponds perfectly to the independently derived amino acid sequence of the human zeta-globin chain. Comparison of the nucleotide sequence of human zeta-globin cDNA with that of human alpha-globin cDNA confirmed previous estimates of very distant evolutionary divergence between the human zeta- and alpha-globin genes. Nevertheless, the human zeta-globin cDNA sequence shares a remarkable similarity to that of the alpha-globin gene in its codon usage, high G + C base composition, and lack of bias against usage of CG dinucleotides.
通过与人α-珠蛋白cDNA探针的交叉杂交检测，从人红白血病细胞系K562的mRNA所构建的cDNA文库中分离出了人胚胎α样ζ-珠蛋白cDNA克隆。对这些cDNA克隆进行核苷酸序列分析，结果显示其编码序列与独立推导得到的人ζ-珠蛋白链氨基酸序列完全一致。将人ζ-珠蛋白cDNA的核苷酸序列与人α-珠蛋白cDNA的核苷酸序列进行比较，证实了先前关于人ζ-珠蛋白基因和α-珠蛋白基因在进化上存在非常遥远分歧的估计。然而，人ζ-珠蛋白cDNA序列在密码子使用、高G + C碱基组成以及对CG二核苷酸使用无偏好性方面，与人α-珠蛋白基因序列具有显著的相似性。
