Wilson J T, Wilson L B, Reddy V B, Cavallesco C, Ghosh P K, deRiel J K, Forget B G, Weissman S M
J Biol Chem. 1980 Apr 10;255(7):2807-15.
The nucleotide sequence of the coding portion of human alpha globin mRNA has been determined by sequence analysis using human alpha globin cDNA cloned in bacterial plasmids. The sequence was obtained by a combination of direct sequence analysis of the cloned cDNA and analysis of cDNA obtained by primer extension, using short restriction endonuclease fragments of cloned alpha cDNA that were hybridized to human globin mRNA and elongated on the mRNA template by viral reverse transcriptase. The human alpha globin mRNA has an unexpectedly high G + C base composition (64.7%), similar to that observed for rabbit globin alpha mRNA, and displays a striking bias in the use of synonym codons for various amino acids. The bias in codon usage of human alpha globin mRNA is similar, with some exceptions, to that previously observed for rabbit alpha globin mRNA as well as for human and rabbit beta globin mRNAs. A detailed restriction endonuclease map of the human alpha globin cDNA is presented.
通过对克隆于细菌质粒中的人α珠蛋白cDNA进行序列分析，已确定了人α珠蛋白mRNA编码部分的核苷酸序列。该序列是通过对克隆的cDNA进行直接序列分析以及对通过引物延伸获得的cDNA进行分析而得到的，使用克隆的α cDNA的短限制性内切酶片段与人类珠蛋白mRNA杂交，并通过病毒逆转录酶在mRNA模板上进行延伸。人α珠蛋白mRNA具有出人意料的高G + C碱基组成（64.7%），类似于在兔珠蛋白α mRNA中观察到的情况，并且在各种氨基酸的同义密码子使用上表现出显著的偏向性。人α珠蛋白mRNA的密码子使用偏向性与之前在兔α珠蛋白mRNA以及人和兔β珠蛋白mRNA中观察到的情况相似，但也有一些例外。本文给出了人α珠蛋白cDNA的详细限制性内切酶图谱。
