用聚乙二醇使紫外线照射过的中国仓鼠细胞透化，并引入来自藤黄微球菌的紫外线核酸内切酶。
Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.
作者信息
Yarosh D B, Setlow R B
机构信息
Biology Department, Brookhaven National Laboratory, Upton, New York 11973.
出版信息
Mol Cell Biol. 1981 Mar;1(3):237-44. doi: 10.1128/mcb.1.3.237-244.1981.
Abstract
Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.
摘要
用聚乙二醇处理中国仓鼠V - 79细胞，使其具有通透性，然后与含有紫外线特异性内切核酸酶活性的藤黄微球菌提取物一起孵育。这种处理在受辐照的而非未受辐照的脱氧核糖核酸中引入了切口。这些切口至少保持开放3小时；没有内切核酸酶敏感位点的丢失，通过色谱法检测也未发现二聚体的切除。此外，处理后的细胞对紫外线的抗性没有增加。这表明啮齿动物细胞中缺乏大量切除修复是由于缺乏切口和切除能力。
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