Kim K, Groman N B
J Bacteriol. 1965 Dec;90(6):1557-62. doi: 10.1128/jb.90.6.1557-1562.1965.
Kim, K. (University of Washington, Seattle), and N. B. Groman. Mode of inhibition of diphtheria toxin by ammonium chloride. J. Bacteriol. 90:1557-1562. 1965.-The inhibition of diphtheria toxin by ammonium salts was independent of toxin concentration over a 100-fold range of toxin. Inhibition by minimal concentrations of ammonium chloride was abolished by lowering the pH, indicating that free ammonia is the active form of inhibitor. A single addition of ammonium chloride inhibited toxin for a limited period of time, but periodic readdition of the ammonium salt was required to sustain inhibition indefinitely in the absence of antitoxin. Toxin was not destroyed and its adsorption occurred equally well in the presence or absence of ammonium chloride. Preadsorbed toxin was also effectively inhibited by the addition of ammonium chloride. Inhibited toxin remained accessible to antitoxin neutralization. Attempts to reverse ammonia inhibition by the addition of succinate or reduced nicotinamide adenine dinucleotide were unsuccessful. Attempts to inhibit toxin by interfering with active transport were also unsuccessful.
金，K.（华盛顿大学，西雅图）和N. B. 格罗曼。氯化铵对白喉毒素的抑制模式。《细菌学杂志》90:1557 - 1562。1965年。——在毒素浓度相差100倍的范围内，铵盐对白喉毒素的抑制作用与毒素浓度无关。降低pH值可消除最低浓度氯化铵的抑制作用，这表明游离氨是抑制剂的活性形式。单次添加氯化铵可在有限时间内抑制毒素，但在没有抗毒素的情况下，需要定期重新添加铵盐以无限期维持抑制作用。毒素未被破坏，其吸附在有或没有氯化铵的情况下同样良好。预先吸附的毒素也能被添加的氯化铵有效抑制。被抑制的毒素仍可被抗毒素中和。通过添加琥珀酸或还原型烟酰胺腺嘌呤二核苷酸来逆转氨抑制的尝试未成功。通过干扰主动转运来抑制毒素的尝试也未成功。
