Lipton J M, Link N A, Breard J, Jackson P L, Clarke B J, Nathan D G
J Clin Invest. 1980 Jan;65(1):219-23. doi: 10.1172/JCI109653.
To determine a possible role of peripheral blood monocytes in erythroid differentiation, various fractions of peripheral blood mononuclear cells were prepared from normal volunteers. The fractions contained 3-95% monocytes. These freshly prepared monocytes did not inhibit erythroid burst forming unit expression in plasma clot erythroid colony culture. Null cell preparations contaminated by up to 84% monocytes expressed erythroid burst forming unit colony formation when either T lymphocytes or T-cell conditioned medium was added. These results indicate that certain peripheral blood null cells engage the program of erythroid differentiation in the presence of T cells and erythropoietin. Monocytes do not inhibit this engagement.
为了确定外周血单核细胞在红系分化中可能发挥的作用，从正常志愿者中制备了外周血单个核细胞的不同组分。这些组分含有3%至95%的单核细胞。这些新鲜制备的单核细胞在血浆凝块红系集落培养中并不抑制红系爆式集落形成单位的表达。当添加T淋巴细胞或T细胞条件培养基时，被高达84%的单核细胞污染的裸细胞制剂表达了红系爆式集落形成单位集落形成。这些结果表明，某些外周血裸细胞在T细胞和促红细胞生成素存在的情况下启动红系分化程序。单核细胞并不抑制这种启动。
