Metcalf E S, Scher I, Klinman N R
J Exp Med. 1980 Feb 1;151(2):486-91. doi: 10.1084/jem.151.2.486.
Previous studies from this laboratory have indicated that the susceptibility to in vitro tolerance induction is restricted to B cells early in their development (12,14). In this study, a modification of the in vitro splenic focus technique was used to determine whether 2,4-dinitrophenyl (DNP)-specific splenic B cells from adult (CBA/N X DBA/2)F1 males are susceptible to in vitro tolerance induction. The results demonstrate that greater than 50% of the DNP-specific B cells in the adult F1 male are tolerizable and therefore immature by this criterion. Moreover, the findings define at least two subpopulation in adult CBA/N mice, one of which is tolerizable. These findings are consistent with the hypothesis that the lymphoid population in the adult CBA/N mouse is characteristic of a neonatal B-cell population.
该实验室先前的研究表明,体外耐受诱导的易感性在B细胞发育早期仅限于B细胞(12,14)。在本研究中,采用体外脾集落技术的改良方法来确定成年(CBA/N×DBA/2)F1雄性小鼠中对2,4-二硝基苯基(DNP)特异性的脾B细胞是否易受体外耐受诱导。结果表明,成年F1雄性小鼠中超过50%的DNP特异性B细胞可被诱导耐受,因此根据这一标准是未成熟的。此外,这些发现定义了成年CBA/N小鼠中至少两个亚群,其中一个是可被诱导耐受的。这些发现与成年CBA/N小鼠中的淋巴细胞群体具有新生B细胞群体特征这一假设一致。