Zigterman G J, Schotanus K, Ernste E B, Van Dam G J, Jansze M, Snippe H, Willers J M
Department of Immunology, State University of Utrecht, The Netherlands.
Infect Immun. 1989 Sep;57(9):2712-8. doi: 10.1128/iai.57.9.2712-2718.1989.
Nonionic block polymer surfactants (NBPs) were tested for the capacity to stimulate the antibody response against hexasaccharide (HS), derived from Streptococcus pneumoniae type 3 capsular polysaccharide (S3PS), which was conjugated to proteins. The immune response was evaluated in the (CBA/N x BALB/c)F1 progeny, in which female mice are phenotypically normal whereas male mice carry an X-chromosome-linked immunodeficiency. NBPs L101, L121, 1101, and 1501 were able to increase anti-HS immunoglobulin M (IgM) and IgG levels in both normal and X-chromosome-linked immunodeficient mice (with up to 74-fold stimulation of antibody titers). Distribution of S3PS-specific antibodies over the various IgG isotypes was restricted after immunization with either HS-bovine serum albumin or HS-keyhole limpet hemocyanin (HS-KLH). Addition of NBPs (in particular 1501) resulted in a more diverse immune response with either antigen as judged by isotype distribution. Isoelectric focusing of individual sera and subsequent detection of S3PS-binding antibodies in these sera by immunochemical staining revealed a restricted number of different spectrotypes in the course of the immune response. Upon immunization of mice with HS-KLH, spectra of secreted antibodies were slightly more complex and more densely stained than after immunization with HS-bovine serum albumin. Furthermore, NBPs 1101 and 1501 appeared to be able to stimulate the secretion of antibodies, which were secreted only in small amounts without the use of NBPs. Different explanations for increased spectrotype diversity after immunization with KLH as the carrier and after administration of NBPs as the adjuvant are discussed.
对非离子嵌段聚合物表面活性剂（NBP）刺激针对六糖（HS）的抗体反应的能力进行了测试，该六糖源自与蛋白质偶联的3型肺炎链球菌荚膜多糖（S3PS）。在（CBA/N×BALB/c）F1后代中评估免疫反应，其中雌性小鼠表型正常，而雄性小鼠携带X染色体连锁免疫缺陷。NBP L101、L121、1101和1501能够增加正常和X染色体连锁免疫缺陷小鼠体内的抗HS免疫球蛋白M（IgM）和IgG水平（抗体滴度刺激高达74倍）。用HS-牛血清白蛋白或HS-钥孔戚血蓝蛋白（HS-KLH）免疫后，S3PS特异性抗体在各种IgG同种型中的分布受到限制。添加NBP（特别是1501）导致以同种型分布判断的对任何一种抗原的免疫反应更加多样化。对个体血清进行等电聚焦，随后通过免疫化学染色检测这些血清中的S3PS结合抗体，结果显示在免疫反应过程中不同光谱类型的数量有限制。在用HS-KLH免疫小鼠后，分泌抗体的光谱比用HS-牛血清白蛋白免疫后略复杂且染色更密集。此外，NBP 1101和1501似乎能够刺激抗体分泌，而在不使用NBP的情况下这些抗体仅少量分泌。讨论了以KLH作为载体免疫和以NBP作为佐剂给药后光谱类型多样性增加的不同解释。
