Huang S C, Phelps M E, Hoffman E J, Sideris K, Selin C J, Kuhl D E
Am J Physiol. 1980 Jan;238(1):E69-82. doi: 10.1152/ajpendo.1980.238.1.E69.
A method for the determination of local cerebral metabolic rates of glucose (LCMRGlc) in normal man is described. The method employs [18F]2-fluoro-2-deoxy-D-glucose (FDG) and emission-computed tomography (ECT). FDG was injected intravenously as a bolus. Radioactivities in separate brain regions were measured with ECT. Plasma FDG concentration following injection was measured from blood samples. A mathematical model that describes the kinetics of FDG transports was employed to determine the transport rate constants of FDG and to convert the radioactivity measurements to metabolic rates. The model has taken into account the possible dephosphorylation reaction from FDG-6-PO4 (FDG-6-P) to free FDG in brain tissues. Experiments were performed in 13 normal volunteers. The rate constants of FDG in man were found to be comparable to those of deoxyglucose in rat and in rhesus monkey. The average LCMRGlc in gray and in white matter were found to be 7.30 +/- 1.18 (SD) and 3.41 +/- 0.64 mg/min per 100 g brain tissue, respectively. The subject-to-subject variation of LCMRGlc as measured by the present method was comparable to those of other methods that measure whole-brain CMRGlc.
本文描述了一种测定正常人局部脑葡萄糖代谢率（LCMRGlc）的方法。该方法采用[18F]2-氟-2-脱氧-D-葡萄糖（FDG）和发射计算机断层扫描（ECT）。FDG以团注方式静脉注射。用ECT测量不同脑区的放射性。从血样中测量注射后血浆FDG浓度。采用描述FDG转运动力学的数学模型来确定FDG的转运速率常数，并将放射性测量值转换为代谢率。该模型考虑了脑组织中从FDG-6-磷酸（FDG-6-P）到游离FDG可能的去磷酸化反应。对13名正常志愿者进行了实验。发现人脑中FDG的速率常数与大鼠和恒河猴中脱氧葡萄糖的速率常数相当。灰质和白质中的平均LCMRGlc分别为每100 g脑组织7.30±1.18（标准差）和3.41±0.64 mg/min。用本方法测量的LCMRGlc在受试者之间的变化与其他测量全脑CMRGlc的方法相当。
