Askonas B A, Webster R G
Eur J Immunol. 1980 Feb;10(2):151-56. doi: 10.1002/eji.1830100215.
The target antigens recognized by the cross-reactive population of cytotoxic T cells on A/USSR/90/77 (H1N1) influenza virus-infected cells was studied with monoclonal antibodies to the H-2k antigen and viral hemagglutinin. The cytotoxic killing of virus-infected cells was differentially inhibited by three monoclonal antibodies to H-2k. Synergistic inhibition of cytotoxic activity was obtained with monoclonal antibodies to H-2 and to some antigenic determinants on the hemagglutinin melecule of A/USSR but not with others. Since target cells infected with other subtypes of influenza A viruses were not inhibited by any of the monoclonal antibodies to A/USSR hemagglutinin, it is suggested that the sites recognized by the cross-reactive cytotoxic T cells were sterically inhibited by the anti-A/USSR monoclonal antibodies. The results suggest that at least one of the target antigens recognized by the cross-reactive population of cytotoxic T cells is located on the hemagglutinin molecule. The present observations are in agreement with cytotoxic T cell recognition of closely situated viral and H-2 determinants.
利用针对H-2k抗原和病毒血凝素的单克隆抗体，研究了A/USSR/90/77（H1N1）流感病毒感染细胞上细胞毒性T细胞交叉反应群体所识别的靶抗原。三种针对H-2k的单克隆抗体对病毒感染细胞的细胞毒性杀伤有不同程度的抑制作用。针对H-2以及A/USSR血凝素分子上某些抗原决定簇的单克隆抗体可协同抑制细胞毒性活性，但其他单克隆抗体则不能。由于感染甲型流感病毒其他亚型的靶细胞未被任何针对A/USSR血凝素的单克隆抗体抑制，提示交叉反应性细胞毒性T细胞所识别的位点在空间上被抗A/USSR单克隆抗体抑制。结果表明，细胞毒性T细胞交叉反应群体所识别的靶抗原中至少有一个位于血凝素分子上。目前的观察结果与细胞毒性T细胞对紧密相邻的病毒和H-2决定簇的识别一致。
